煤绒菌(Fuligo sp.)DNA提取方法的研究及用于扩增EF1A基因片段的引物设计

Extraction Methods of Genomic DNA from Fuligo sp.and Primers Design for Elongation Factor

采用氯化苄法提取煤绒菌的基因组DNA,并成功获得PCR产物。同时采用Primer 5.0软件设计得到延长因子EF1A特异性引物EF243、ER824和ER1001,用这些引物对煤绒菌的延长因子EF1A基因进行半巢式PCR扩增获得成功。

Genomic DNA was first extracted using benzyl chloride,and PCR product was successfully acquired.Meantime,the specific primers to EF1A gene,EF243,ER824 and ER1001 were designed using Primer 5.0 software,and the EF1A gene of Fuligo sp.was successfully amplified by semi-nested PCR using these primers.