鲜人参及其加工品中腺苷和L-焦谷氨酸含量比较研究

Comparative Study on Adenosine and L-pyroglutamic Acid Content of Fresh Ginseng and Its Processed Products

以六年生鲜人参、生晒参、红参为试验材料,建立腺苷和L-焦谷氨酸的含量测定方法：RP-HPLC法分析样品中腺苷含量,依利特Hypersil ODS2（4.6mm×250 mm,5μm）色谱柱,流动相为甲醇—水（10∶90）,流速0.8 mL/min,检测波长260 nm,柱温40℃;RP-HPLC法分析样品中L-焦谷氨酸含量,COSMOSIL 5C18-PAQ（4.6mm×250mm,5μm）色谱柱,流动相为甲醇—5 mmol/L醋酸钠水溶液（8∶92）,流速0.5 mL/min,检测波长210 nm,柱温40℃。测得鲜人参及其加工品中腺苷含量为0.332~0.457 mg/g,L-焦谷氨酸含量为0.315~1.056 mg/g。结果表明：腺苷和L-焦谷氨酸含量在鲜人参及其加工品中呈规律性变化,鲜人参中腺苷和L-焦谷氨酸含量最高,加工品次之。因此在抗糖尿病活性方面,鲜人参较其加工品应更具研究价值。

This study is to establish the methods for assaying adenosine and L-pyroglutamic acid in ginseng.Six-year-old fresh ginseng,sun-dried ginseng and red ginseng were used as materials.RP-HPLC was used to analyze adenosine in samples.Separation was carried out using a chromatographic column（Hypersil ODS2,4.6 mm×250 mm,5 μm）.Column temperature and detection wavelength were set at 40 ℃ and 260 nm,respectively.Mobile phase containing methanol-water（10∶90） was used and the flow rate was 0.8 mL/min.Chromatographic column（COSMOSIL 5C18-PAQ,4.6 mm×250 mm,5 μm） was applied to analyze L-pyroglutamic acid in ginseng.Mobile phase contained methanol-sodium acetate water solution of 5 mmol/L（8∶92）,and the flow rate was 0.5 mL/min.Column temperature and detection wavelength for analyzing L-pyroglutamic acid were set at 40 ℃ and 210 nm,respectively.Adenosine in fresh ginseng and its processed products ranged from 0.332 mg/g to 0.457 mg/g,the amount of L-pyroglutamic acid ranged from 0.315 mg/g to 1.056 mg/g.The results showed that： the amount of adenosine and L-pyroglutamic acid showed regular changes in fresh ginseng and its processed products.Substances of adenosine and L-pyroglutamic in fresh ginseng showed the maximum in different ginseng products,processed products was secondary.Therefore,fresh ginseng possesses more research value than its processed products for anti-diabetic activity.