血管紧张素原基因-20A/C多态性与2型糖尿病肾病相关性研究

Study on relationship between-20A/C angiotensinogen gene polymorphisms and diabetic nephropathy of T2DM patients

目的探讨血管紧张素原(AGT)基因-20A/C多态性与DN关系。方法应用聚合酶链反应-限制性片段长度多态性分析法(PCR-RFLP)对292例T2DM患者(正常白蛋白尿组148例,微量白蛋白尿组105例,大量白蛋白尿组39例)及157名正常对照(NC)者进行AGT基因-20A/C多态性检测。结果男性大量白蛋白尿组等位基因C频率高于正常白蛋白尿组(P=0.046)。Logistic回归分析显示,携带等位基因C(OR:4.268,P=0.043,95%CI:1.044～17.447)与男性大量白蛋白尿相关。结论 AGT基因-20A/C多态性可能与浙江地区汉族人群男性T2DM患者出现大量白蛋白尿相关。

Objective To investigate the relationship between angiotensinogen（AGT） gene-20A/C polymorphism and the susceptibility to type 2 diabetic nephropathy.Methods Using polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） to detect AGT gene-20A/C polymorphisms in 292 patients with T2DM（148 in normo-albuminuria group,105 in micro-albuminuria group,and 39 in macro-albuminuria group） and 157 normal controls（NC）.Results In the male macro-albuminuria group,the frequency of C allele was significantly higher than in the normo-albuminuria group（P=0.046）.The AGT gene-20A/C polymorphism（OR： 4.268,P=0.043,95%CI： 1.044~17.447） was an independent risk factor for macro-albuminuria in male patients.Conclusion AGT gene-20A/C polymorphism might be a susceptibility gene for macro-albuminuria in male T2DM patients in Zhejiang Han population.