2型糖尿病肾病患者血清中性粒细胞明胶酶相关脂质运载蛋白检测及意义的观察

Detection and significance of serum neutrophil gelatinase-associated lipocalin in patients with type 2 diabetic nephropathy

目的探讨DN患者血清中性粒细胞明胶酶相关脂质运载蛋白(NGAL)的变化及临床意义。方法应用ELISA法检测血清NGAL水平,同时测BMI、FPG、HbA1c、高敏C反应蛋白(hs-CRP)、血脂及UAER,比较各组间的差异。结果 T2DM各组血清NGAL水平均较NC组明显升高(P<0.01)。相关分析显示,血清NGAL水平与Cr、UAER、hs-CRP、TC、HDL-C呈正相关,r分别为0.664、0.877、0.726、0.300、0.578,P<0.01;与FPG呈正相关,r为0.180,P<0.05;与HbA1c呈负相关,r为-0.172,P<0.01。多元逐步回归分析显示,与血清NGAL水平密切相关的因素为UAER、hs-CRP。结论 DN患者血清NGAL水平明显升高,且血清NGAL水平与UAER、hs-CRP密切相关。提示NGAL与早期肾损害程度相关。

Objective To investigate the changes and clinical significance of serum neutrophil gelatinase-associated lipocalin（NGAL） in diabetic nephropathy（DN）.Methods A total of 99 T2DM patients and 30 healthy people as control were enrolled in the study.According to UAER,the diabetic patients were divided into groups NA（normal albuminuria,n=32）,MA（microalbuminuria,n=39）,and CN（clinical nephropathy,n=28）.NGAL level was detected by ELISA.BMI,FPG,HbA1c,hs-CRP,blood lipid,and UAER were measured as well.Data were compared among groups.Results The serum NGAL level was higher in T2DM patients than in the healthy control group（P 0.01）.Correlation analysis indicated that serum NGAL level was positively correlated with Cr（r=0.664）,UAER（r=0.877）,hs-CRP（r=0.726）,TC（r=0.300）,and HDL-C（r=0.578）（P0.01）,and FPG（r=0.180）（P 0.05）;while negatively with HbA1c（r=-0.172）（P0.01）.Multiple regression analysis showed that UAER and hs-CRP were closely associated with the level of serum NGAL.Conclusion The serum NGAL level in patients with DN significantly increases and is closely correlated with UAER and hs-CRP.It suggests an association of NGAL level with early renal damage.