液相色谱串联质谱法测定猪尿中9种β-受体激动剂残留

Detection of 9 β-agonists multiresidue in pig urine by liquid chromatography-mass spectrometry

参照中国农业部1025号公告-11-2008的常规前处理方法,采用新的检测条件,以液相色谱串联质谱法对猪尿样本中9种β-受体激动剂实行同时检测。并在内标物质有限的情况下,根据色谱条件,为没有内标的化合物灵活地选择合适的内标物质。β-受体激动剂添加浓度为1,2,4,10,20ng/mL时,平均回收率为82.12%～118.61%,相对标准偏差为0.97%～11.53%,检测限在0.01～0.12ng/mL,其回收率和灵敏度高,线性范围广,分析精度好,并能提高原有方法的检测水平。

A LC MS method is presented to simultaneously analyze 9 agonists in pig urine with a new detecting condition and a conven- tional sample pretreatment method which is commonly used, prom ulgated in 1025 by Ministry of Agriculture in China. And according to chromatographic conditions, the limited internal standards were used flexibly for several target compounds. Calibration plots of 9 agonists were linear from 1 to 20 ng/mL. The average recoveries ranged between 88.31% and 118.61%, with relative standard devia- tions of 0. 97-11. 53%. The limits of detection were 0. 01 0.12 ng/mL. The method has the capacity for detecting 9 β-agonists with high accuration, sensitivity, recovery and a wide range of line- arity.