两面针红外指纹图谱与抗癌活性的谱效研究

Infrared fingerprint of Zathoxylum nitidum and its effect on inhibition of tumor cell

研究两面针的红外指纹图谱与抗肿瘤活性之间的相互关系.基于不同产地的两面针氯仿提取物的红外指纹图谱特征峰强度及其抗肿瘤活性的效果,采用后退法构建以两面针抑制人胃腺癌7901和人宫颈癌Hela两种肿瘤细胞株的谱效模型.所建立数学模型的预测值与实际测量值的偏差率全部在10%以内,说明两面针红外指纹图谱和两面针抗肿瘤活性之间具有相关性.结果表明,生物碱类成分在两面针抗肿瘤药效活性中发挥了重要的作用.

Mathematical expression was established for evaluating anticancer efficacy of samples of Z. nitidum to human tumor cells based on their infrared fingerprints. Chloroform extracts of the root of Z. nitidum from different habitats in China were used as subject samples to do anticancer experiments on two strains of human tumor cells, 7901 and Hela cells. Meanwhile, their fingerprints of infrared spectra were obtained. Screening arguments of backward elimination method was adopted to establish the spectra-effect correlation between infrared fingerprint and pharmacology data. The results showed that deviations of the predictive values from the true values were within ± 10% for the two kinds of tumor cells, suggested that there was close correlation between infrared fingerprint and biological activity of Z. nitidum. The model implied that alkaloids in Z. nitidum have main effect on tumor cells.