玉簪属植物花瓣中类黄酮化合物价值评价

Flavonoid compounds in the petals of Hosta

为拓宽玉簪属植物除园林方面的应用,本研究以玉簪属5个野生种和85个栽培品种的花瓣为材料,利用高效液相色谱(HPLC-DAD)技术,建立了定性定量分析玉簪花中类黄酮化合物的HPLC方法,可作为玉簪花药材的质量控制方法。同时结合质谱(MSn)技术,鉴定了玉簪花瓣中17种类黄酮化合物的化学结构,分别为:山奈酚3,7-二葡萄糖苷、山奈酚3-葡萄糖-7-芸香糖苷、异鼠李素3,4-二葡萄糖苷、山奈酚3-奎尼酰葡萄糖苷、山奈酚3-芥子酰葡萄糖苷、山奈酚3-抗坏血酰葡萄糖苷、山奈酚7-新橙皮糖苷、山奈酚3,7-二己糖苷、山奈酚3-葡萄糖-7-鼠李糖苷、木犀草素7-新橙皮糖苷、槲皮素3-葡萄糖苷、槲皮素3-葡糖苷酸、槲皮素3-刺槐二糖苷、山奈酚3-葡萄糖苷、山奈酚3-葡糖苷酸、山奈酚3-戊糖苷和山奈酚7-葡萄糖苷。并比较了90种玉簪花中类黄酮化合物成分与含量的差异性,筛选出类黄酮化合物含量较高的种类。

The flavonoid composition of five Hosta species and 85 Hosta cultivars was investigated by HPLC-DAD for the first time to expand the applications of Hosta.Qualitative and quantitative analysis methods for total flavonoids in flowers of Hosta were used as a basis for the quality control of Hosta.A combination of powerful tools（HPLC-DAD and HPLC-MSn）,were used to characterize seventeen kinds of flavonoid including kaempferol 3,7-di-O-glucoside,kaempferol 3-O-glucosyl-7-O-rutinose,isorhamnetin 3,4-di-O-glucoside,kaempferol 3-O-quinoylglucoside,kaempferol 3-O-sinapoylglucoside,kaempferol 3-O-ascorbicoylglucoside,kaempferol 7-O-neohesperidoside,kaempferol 3,7-di-O-hexoside,kaempferol 3-O-glucosyl-7-O-rhamnoside,luteolin 7-O-neohesperidoside,quercetin 3-O-glucoside,quercetin 3-O-glucuronide,kaempferol 3-O-robinobioside,kaempferol 3-O-glucoside,kaempferol 3-O-glucuronide,kaempferol 3-O-pentose,and kaempferol 7-O-glucoside.The flavonoid contents of 90 kinds of Hosta flowers were compared and the high-value species were selected.