正交试验法优选滇丹参中水溶性成分提取工艺

Study on Extracting Process of Water Soluble Component of Salvia yunnanensis C. H. Wright

目的用正交试验法优选滇丹参水溶性有效成分丹酚酸B的最佳提取工艺。方法以高效液相色谱法测得的丹酚酸B的含量和提取物出膏率为指标,采用以乙醇浓度、加醇量、提取时间、提取次数为影响因素的正交试验法,对滇丹参中丹酚酸B的提取工艺进行优选。结果滇丹参中水溶性成分丹酚酸B的最优提取工艺为8倍量20%乙醇回流提取3次,每次1h。结论此工艺稳定可行,可为滇丹参水溶性有效成分的提取提供实验依据。

Objective To optimize the extracting process of the water-soluble active component of Salvia yunnanensis C. H. Wright by orthogonal design. Methods The extracting process was studied by orthogonal design,which includes ethanol concentration and alcohol amount and extracting hours and extracting times,with the content of salvianolic acid B by RP-HPLC and the yield of extraction of the Salvia yunnanensisC. H. Wright. as the indexes for screening the extracting condition. Results The best extraction condition of salvianolic acid B from Salvia yunnanensis C. H. Wright. was：adding eight times 20% ethyl alcohol,extracting for 3 times,each time lbr. Conclusion The optimized extracting process for salvianolic acid B from Salvia yunnanensisC. H. Wright was satable and feasible with operability,and can provide experimental evidence for the extraction of the water-soluble active component of Salvia yunnanensisC. H. Wright.