饥饿胁迫对卵形鲳鲹幼鱼消化器官组织学的影响

Effects of Starvation Stress on the Histological Structure of the Digestive Organs for Juveniles of Trachinotus ovatus

将375尾卵形鲳鲹Trachinotus ovatus幼鱼分别饥饿0、3、6、9和12 d,利用形态学和连续组织切片技术,观察和研究了饥饿胁迫对卵形鲳鲹幼鱼食道、胃、幽门盲囊、肠和肝胰脏等消化器官组织结构的影响,了解其在饥饿状态下的形态和组织学结构变化及耐受饥饿的能力。3 d以内的短时间饥饿对消化器官组织结构的影响不明显,随着饥饿时间延长,消化器官开始出现损伤,饥饿6～9 d对消化器官的损伤程度较轻,饥饿12 d消化器官损伤严重,出现消化道管腔变窄、变薄,黏膜上皮细胞界限变得模糊,上皮逐渐脱落、断裂,分泌细胞变小等。食道组织结构受饥饿胁迫的损伤程度较其他消化器官轻,胃、幽门盲囊和肠道的组织结构受饥饿的影响相似,肝胰脏受饥饿的影响最严重。结果表明,饥饿胁迫使卵形鲳鲹幼鱼的消化器官发生了不同程度的变化,不同的饥饿胁迫时间对不同消化器官的影响程度也不同。

By using morphorlogy and paraffin section technique, effects of starvation stress on the histo- logical structure of the digestive organs including esophagus, stomach, pyloric caecum, intestine and hepatopancreas and their characteristics of changes were investigated in 375 juveniles of Trachinotus ova- tus starved for O, 3, 6, 9 and 12 d respectively for the purpose of understanding the changes in morphorl- ogy and histology and capacity of tolerence under starvation. The histology results show that effects of the short time starvation within 3 d on the digestive system were of no significance, with starvation time injury began to occur in digestive organs. The damage to digestive organs was lesser from the 6 to 9th days after starvation, and on 12th days after starvation, severe damage were found. Lumen of digestive tract became narrow and thinning, the epithelial cell boundaries were blurred , epithelial falled off and fractured, and secretory cells bacame smaller in size. Damage of histological structure in esophagus by starvation were minor compared with other organs. The effects of starvation were similar about the histological structure of the stomach, pyloric caeca and intestinal tract. Severer damage were found in hepatopancreas than other organs. In conclusion changes to varying degrees took place in digestive organs of juveniles of T. ovatus by starvation, and effect of starvation on differenct organs varied as time.