傲骨胶囊对去卵巢大鼠骨质疏松相关检测指标的影响

Effect of AOGU CAPSULE on the detection indicators related to the osteoporosis of ovariectomized rats

目的:观察傲骨胶囊对去卵巢大鼠骨质疏松相关检测指标的影响。方法:将50只健康10周龄雌性SD大鼠分为5组,A组9只,B组10只,C组10只,D组11只,E组10只。A组大鼠切除卵巢周围部分脂肪,其余4组切除卵巢制成骨质疏松模型。从造模后第11天开始,C组、D组、E组大鼠分别按0.3375g.kg-1、0.675g.kg-1、2.025g.kg-1以傲骨胶囊灌胃,A组和B组按10mL.kg-1以去离子水灌胃,每天1次,共13周,第13周末处死大鼠。从药物干预开始至处死前,每周测定1次各组大鼠的体质量。取各组大鼠左侧股骨,测定其股骨中点、远端和近端骨密度。取各组大鼠右侧股骨,测定其骨钙含量和骨恒重。结果:①体质量。药物干预前5组大鼠体质量比较,差异有统计学意义(F=7.230,P=0.000)。组间两两比较:A组大鼠体质量[(241.40±19.10)g]小于B组[(281.90±21.00)g]、C组[(280.70±22.60)g]、D组[(282.20±20.90)g]和E组[(281.70±23.40)g],差异均有统计学意义(P=0.000,P=0.000,P=0.000,P=0.000)。药物干预13周后5组大鼠体质量均有增加(t=4.180,P=0.000;t=15.390,P=0.000;t=12.140,P=0.000;t=15.590,P=0.000;t=11.630,P=0.000),对增加值进行组间比较,差异有统计学意义(F=7.364,P=0.000)。进一步组间两两比较:A组增加值[(100.20±39.25)g]小于B组[(178.10±36.78)g]和D组[(143.10±29.18)g],差异均有统计学意义(P=0.000,P=0.008);B组增加值大于C组[(116.20±30.43)g]、D组和E组[(130.30±35.63)g],差异均有统计学意义(P=0.000,P=0.028,P=0.003)。其余各组间两两比较,差异无统计学意义(P>0.05)。②骨密度。5组大鼠股骨远端、股骨中心点及股骨近端骨密度组间比较,差异均有统计学意义(F=15.549,P=0.000;F=4.688,P=0.005;F=4.343,P=0.005)。B组股骨远端、股骨中心点及股骨近端骨密度[(0.21±0.02)g.cm-2,(0.13±0.02)g.cm-2,(0.18±0.03)g.cm-2]均低于A组[(0.27±0.02)g.cm-2,(0.16±0.01)g.cm-2,(0.22±0.02)g.cm-2]、D组[(0.26±0.02)g.cm-2,(0.16±0.03)g.cm-2,(0.20±0.02)g.cm-2]、E组[(0.26±0.03)g.cm-2,(0.18±0.01)g.cm-2,(0.21±0.01)g.cm-2],差异均有统计学意义(P=0.000,P=0.001,P=0.000;P=0.000,P=0.001,P=0.039;P=0.000,P=0.000,P=0.003);C组股骨远端和股骨近端骨密度[(0.22±0.01)g.cm-2,(0.18±0.02)g.cm-2]低于D组,差异均有统计学意义(P=0.000,P=0.039);C组股骨远端、股骨中心点[(0.15±0.02)g.cm-2]及股骨近端骨密度均低于E组(P=0.000,P=0.001,P=0.003);D组股骨中心点骨密度低于E组(P=0.027);其余各组间比较,差异均无统计学意义(P>0.05)。③骨钙含量。5组大鼠骨钙含量比较,差异有统计学意义(F=5.929,P=0.001)。A组骨钙含量[(254.12±19.11)mg.g-1]大于B组[(203.23±30.58)mg.g-1]和C组[(226.35±26.71)mg.g-1],差异均有统计学意义(P=0.000,P=0.027);B组骨钙含量小于D组[(245.80±16.43)mg.g-1]和E组[(239.40±37.30)mg.g-1],差异均有统计学意义(P=0.001,P=0.005);其余各组间比较,差异均无统计学意义(P>0.05)。④骨恒重。5组大鼠骨恒重比较,差异有统计学意义(F=5.147,P=0.001)。E组大鼠骨恒重[(664.51±44.75)mg]大于A组[(603.77±70.05)mg]、B组[(603.38±36.57)mg]和C组[(611.93±53.03)mg],差异均有统计学意义(P=0.013,P=0.012,P=0.030);其余各组间比较,差异均无统计学意义(P>0.05)。结论:中、高剂量的傲骨胶囊能促进去卵巢大鼠对钙的吸收和利用,增加骨密度,具有明显的抗骨质疏松作用。同时傲骨胶囊还有抑制由雌激素水平降低所引起的体质量增加的作用。

Objective：To observe the effect of AOGU CAPSULE on the detection indicators related to the osteoporosis of ovariectomized rats.Methods：Fifty healthy female SD rats of 10 weeks old were divided into 5 groups,9 cases in group A,10 cases in group B,10 cases in group C,11 cases in group D and 10 cases in group E.Rats in group A were administrated with fat removal around the ovary,while the others in the rest 4 groups were administrated with ovariectomy to build models of osteoporosis.Ten days after molding,the rats in group C,D and E were intragastric administrated with AOGU CAPSULE（0.3375 g/kg,0.675 g/kg and 2.025 g/kg,respectively）,while the others in group A and group B were intragastric administrated with deionized water（10 mL/kg）,once per day for consecutive 13 weeks.All the rats were executed at the end of the 13th week.During the period from the beginning of medicine intervention to execution,the body mass of rats of each group were measured once a week.The bone mineral density（BMD）were measured on the midpoint,distal end and proximal end of the left femur of the rats,and the bone calcium content（BCC）and bone constant weight（BCW）were measured on the right femur of the rats.Results：①Body mass：there was statistical difference in body mass among the 5 groups before medicine intervention（F=7.230,P=0.000）.As the results of further pairwise comparison showed,body mass of rats in group A（（241.40±19.10）g）were lower than that of group B（（281.90±21.00）g）,C（（280.70±22.60）g）,D（（282.20±20.90）g）and E（（281.70±23.40）g）respectively,and there were statistical differences between them（P=0.000,P=0.000,P=0.000,P=0.000）.Body mass of rats in the 5 groups were all increased 13 weeks after medicine intervention（t=4.180,P=0.000;t=15.390,P=0.000;t=12.140,P=0.000;t=15.590,P=0.000;t=11.630,P=0.000）,and there was statistical difference in the increase value among the 5 groups（F=7.364,P=0.000）.As the results of further pairwise comparison showed,the increase value of group A（（100.20±39.25）g）was lower than that of group B（（178.10±36.78）g）and group D（（143.10±29.18）g）respectively,and there were statistical differences between them（P=0.000,P=0.008）;the increase value of group B was larger than that of group C（（116.20±30.43）g）,D and E（（130.30±35.63）g）respectively,and there were statistical differences between them（P=0.000,P=0.028,P=0.003）.There was no statistical difference between any couple of the rest groups（P0.05）.②BMD：there were statistical differences in BMD on distal femur,femoral midpoint and proximal femur among the 5 groups（F=15.549,P=0.000;F=4.688,P=0.005;F=4.343,P=0.005）.BMD on distal femur,femoral midpoint and proximal femur of rats in group B（（0.21±0.02）g/cm（2）,（0.13±0.02）g/cm（2）,（0.18±0.03）g/cm（2））were all lower than those of group A（（0.27±0.02）g/cm（2）,（0.16±0.01）g/cm（2）,（0.22±0.02）g/cm（2））,D（（0.26±0.02）g/cm（2）,（0.16±0.03）g/cm（2）,（0.20±0.02）g/cm（2））and E（（0.26±0.03）g/cm（2）,（0.18±0.01）g/cm（2）,（0.21±0.01）g/cm（2））respectively,and there were statistical differences between them（P=0.000,P=0.001,P=0.000;P=0.000,P=0.001,P=0.039;P=0.000,P=0.000,P=0.003）;BMD on distal and proximal femurs of rats in group C（（0.22±0.01）g/cm（2）,（0.18±0.02）g/cm（2））were all lower than those of group D,and there were statistical differences between them（P=0.000,P=0.039）;BMD on distal femur,femoral midpoint（（0.15±0.02）g/cm（2））and proximal femur of rats in group D were all lower than those of group E respectively（P=0.000,P=0.001,P=0.003）;BMD on femoral midpoint of group D was lower than that of group E（P=0.027）;while there were no statistical differences between any couple of the rest groups（P0.05）.③BCC：there was statistical difference in BCC among the 5 groups（F=5.929,P=0.001）.BCC of group A（（254.12±19.11）mg/g）was larger than that of group B（（203.23±30.58）mg/g）and group C（（226.35±26.71）mg/g）respectively,and there were statistical differences between them（P=0.000,P=0.027）;BCC of group B was lower than that of group D（（245.80±16.43）mg/g）and group E（（239.40±37.30）mg/g）respectively,and there were statistical differences between them（P=0.001,P=0.005）;while there were no statistical differences between any couple of the rest groups（P0.05）.④BCW：there was statistical difference in BCW among the 5 groups（F=5.147,P=0.001）.BCW of group E（（664.51±44.75）mg）was larger than that of group A（（603.77±70.05）mg）,B（（603.38±36.57）mg）and C（（611.93±53.03）mg）respectively,and there were statistical differences between them（P=0.013,P=0.012,P=0.030）;while there were no statistical differences between any couple of the rest groups（P0.05）.Conclusion：AOGU CAPSULE of medium and high dosage can promote the absorption and use of the calcium and increase BMD in ovariectomized rats,so it has significant antagonistic effect on osteoporosis.Meanwhile,AOGU CAPSULE also has the effect of inhibiting the increase of body mass caused by the lower level of estrogen.