猪红细胞的体外双修饰及异源输血试验

Study on in Vitro Dual-modified Pig Red Blood Cells and Heterologous Blood Transfusion

为探讨表面抗原修饰后猪红细胞异源输血的可行性,采用α-半乳糖苷酶和单甲氧基聚乙二醇琥珀酰亚胺碳酸酯(mPEG-SC)对猪红细胞进行双修饰后,体外检测红细胞的渗透脆性,并进行配血试验和犬输血试验。观察输血前后犬的各项临床指标变化,并对其进行血常规和尿常规的连续检测分析。结果表明,修饰红细胞渗透脆性降低,体外配血未出现凝集反应。输血后亦未引起明显的临床异常反应,血常规及尿常规正常。说明猪红细胞体外双修饰后进行首次异源输血具有可行性,可作为动物通用血源用于临床。

To explore the possibility of heterologous blood transfusion to dog after removing surface antigen of pig red blood cells , the osmotic fragility of pig red blood cells modified by a galactosidase glycosidase and mPEG-SC in vitro was detected,and experiment of matching of blood was done. The clinical indicators of dog were observed, and blood and urine routine was detected and analyzed eontinuously hefore and after blood transfusion. The resuhs showed that the modification reduced erythrocyte osmotic fragility and the blood did not appear to agglutination in vitro. The dog had no obvious clinical abnormal reactions after transfusion, and its indexes of blood and urine were normal. Conclusion, It is feasible that pig red blood cells modified are heterologous transfused to dog firstly, and could be used for veterinary clinic as general all purpose blood.