枯草芽孢杆菌的分离筛选

Isolation and Screening of Bacillus Subtilis

本文基于从秸秆垛底层土壤中分离枯草芽孢杆菌的目的,为发酵秸秆提供菌株,从陕西杨凌农村秸秆垛中采取底层土样并进行分离。分离出11株菌,经过形态特征、生理生化特征等方法从中分离初步鉴定了3株疑似菌株。经对液态发酵条件的优化,对3株疑似菌进行液态发酵试验,以粗蛋白和粗纤维含量为标准,筛选出一株发酵效果最好的菌株b-1进行了16SrDNA序列分析,最终鉴定b-1菌株为枯草芽孢杆菌(Bacillus subtilis),其同源性≥99%。

This study aimed to isolate Bacillus subtilis from the bottom soil of wheat straw and provide strains for straw fer- mentation, the soil sample was from Yangling Shaanxi. 11 strains were isolated,3 suspected strains were identified using morphological, physiological and biochemical characteristics methods. After optimizing of liquid fermentation, a best strain b--1 was screened with CP and CF as standard and 16S rDNA sequence was carried out,strain b--1 is considered to be Bacillus subtilis, and shows 99%homology.