中华鳖多态性微卫星标记跨物种扩增研究

The study of cross-species amplification of polymorphic microsatellite markers from Pelodiscus sinensis

微卫星是一种优秀的分子标记,并广泛应用于生物和医学的各个领域。但微卫星标记的开发成本较高,因而,利用近缘种已开发的微卫星标记已成趋势。利用本实验室已开发的21个中华鳖微卫星对鼋、斑鳖、山瑞鳖、刺鳖、佛罗里达鳖、两爪鳖、黄喉拟水龟和乌龟等8个物种进行跨物种检验。结果表明,4个微卫星位点(CST21、CST42、CST50和CST52)的引物能在所有8只个体中稳定扩增;而CST16在所有个体中都不能扩增出目的片段。跨物种扩增山瑞鳖结果最好,21个位点中有20个位点能稳定扩增;而乌龟扩增效果最差,只有6个位点能被扩增出目的产物。总之,中华鳖的21个微卫星位点多数可以在鳖科内各属间进行跨物种扩增,部分可以在跨科物种间扩增。

The microsatellite is an excellent molecular marker, and is widely used in biological and medical fields. The application of microsatellite markers developed from closely related species has become a trend. In this study, cross-species amplification of 21 loci isolated from the species Pelodiscus sinensis （our laboratory developed） was carried out in other eight species （Pelochelys cantorii, Rafetus swinhoei, Palea steindachneri, Apalone spinifera, Apalone ferox, Carettochelys insculpta, Mauremys mutica, Chinemys reevesii ）. The 4 loci （ CST21, CST42, CST50 and CST52） were amplified specific products in all eight species, while CST16 could not be ampli- fied target products in all individuals. The best result of amplification occurred in Palea steindachneri which 20 loci could be amplified stably specific products, while the worst result of amplification occurred in Chinemys reevesii, for only 6 loci could be amplified specific products. Overall, the majority of the 21 microsatellite loci of the Chinese soft-shelled turtle can be used for cross-genera amplification, even some of these loci can be used for cross-families amplification.