氮、磷对单针藻兼养生长与油脂合成的影响

Effects of nitrogen and phosphorus on growth and lipid synthesis of Monoraphidium sp. under mixotrophic cultivation

在兼养条件下,应用Andrew方程考察氮、磷营养盐对单针藻生长的影响。分别计算得到单针藻对磷的半饱和常数与对氮的半饱和常数。结果表明:KSP<KSN说明磷对单针藻生长的影响大于氮;并研究了氮、磷营养盐对单针藻油脂质量分数的影响。结果显示:分别在NaNO3质量浓度为1 g/L时,在K2HPO4.3H2O质量浓度为0.1 g/L时,生物量产率、油脂产率达到最大值,油脂质量分数相对较高。单针藻的油脂产率131.79 mg/(L.d)在绿藻中相对较高。

The effects of nitrogen and phosphorus on growth of Monoraphidium sp. FXY-10 under mixotrophic cultivation were investigated by using Andrew equation. The semi-saturation constants of Monoraphidium sp. FXY-10 to phosphorus Ksp and to nitrogen KsN were calculated. The results show that Ksp 〈 KsN, which means that the effect of phosphorus on the growth of Monoraphidium sp. FXY-10 is greater than that of nitrogen. The effects of nitrogen and phosphorus on lipid content of Monoraphidium sp. FXY-10 were studied. The results reveal that the biomass productivity, the lipid productivity are the highest and the lipid content is higher when the mass concentrations of NAN03 and K2HPO4 3H20 are 1 g/L and 0. 1 g/L, respectively. Under mixotrophic cultivation, the lipid productivity 131.79 mg/（ L d） of Monoraphidium sp. FXY-10 is relatively high in green algae.