小麦幼苗微管结合蛋白MAP65的鉴定和基本特性的研究(英文)

Identification and Preliminary Characterization of Wheat Mesophyll Cells 65 kDa Microtubule-associated Protein

[目的]探讨小麦幼苗MAP65体外对微管聚合的影响。[方法]以"临优2018"小麦幼苗为材料,通过免疫印迹实验鉴定小麦幼苗中微管结合蛋白MAP65的存在,并利用紫外吸收法和SDS-聚丙烯酰胺凝胶电泳法探究了小麦幼苗MAP65体外对微管聚合的影响。[结果]免疫印迹实验表明在小麦幼苗中存在微管结合蛋白MAP65;共沉淀实验结果显示,MAP65蛋白可与微管结合,具有一般微管结合蛋白的基本特性;浊度法实验显示MAP65对微管聚合的影响与其浓度相关,低浓度促进微管聚合,高浓度则抑制微管聚合。[结论]为进一步研究MAP65蛋白的生理功能提供了重要的依据。

[Objective] This study aimed to test the characteristics of MAP65 in wheat mesophyll cells by co-sedimentation experiments. [Method] We used wheat Linyou 2018 as the experimental material. Western blotting analysis was carried out to de- tect the existing of 65 kDa MAP in the wheat mesophyll cells; spectrophotometery and SDS-PAGE were adopted to analyze the role of MAP65 in microtubules poly- merization in vitro. [Result] MAP65 indeed existed in wheat mesophyll cells. Co-sedi- mentation experiments showed that MAP65 can bind to microtubules, with the basic characteristics of the microtubule-associated proteins. Turbidimetric experiments showed that microtubule polymerization depended on the concentration of MAP65. Low concentrations of MAP65 promoted microtubule polymerization while high con- centrations of MAP65 inhibited microtubule polymerization. [Conclusion] This study laid significant basis for further research on physiological function of MAP65 protein.