摘要
[目的]探讨小麦幼苗MAP65体外对微管聚合的影响。[方法]以"临优2018"小麦幼苗为材料,通过免疫印迹实验鉴定小麦幼苗中微管结合蛋白MAP65的存在,并利用紫外吸收法和SDS-聚丙烯酰胺凝胶电泳法探究了小麦幼苗MAP65体外对微管聚合的影响。[结果]免疫印迹实验表明在小麦幼苗中存在微管结合蛋白MAP65;共沉淀实验结果显示,MAP65蛋白可与微管结合,具有一般微管结合蛋白的基本特性;浊度法实验显示MAP65对微管聚合的影响与其浓度相关,低浓度促进微管聚合,高浓度则抑制微管聚合。[结论]为进一步研究MAP65蛋白的生理功能提供了重要的依据。
[Objective] This study aimed to test the characteristics of MAP65 in wheat mesophyll cells by co-sedimentation experiments. [Method] We used wheat Linyou 2018 as the experimental material. Western blotting analysis was carried out to de- tect the existing of 65 kDa MAP in the wheat mesophyll cells; spectrophotometery and SDS-PAGE were adopted to analyze the role of MAP65 in microtubules poly- merization in vitro. [Result] MAP65 indeed existed in wheat mesophyll cells. Co-sedi- mentation experiments showed that MAP65 can bind to microtubules, with the basic characteristics of the microtubule-associated proteins. Turbidimetric experiments showed that microtubule polymerization depended on the concentration of MAP65. Low concentrations of MAP65 promoted microtubule polymerization while high con- centrations of MAP65 inhibited microtubule polymerization. [Conclusion] This study laid significant basis for further research on physiological function of MAP65 protein.
基金
Supported by the National Science Foundation of China (30671061)
the Natural Science of Shanxi Province (2008011059-1)~~
关键词
小麦
微管
微管结合蛋白
MAP65
Wheat mesophyll cells
Taxol
Tubulin
Microtubules
MAP65