9株羊布鲁杆菌的基因突变研究

The study on gene mutation about 9 strains of Brucella melitensis

目的对从血流感染的患者体内分离得到的9株致病菌进行菌属鉴定,从DNA水平分析9株菌与标准菌株的染色体差异。方法常规方法确定布鲁氏菌属,用分子生物学技术克隆9株布鲁氏菌的致病基因OMP25、OMP31、UGPB及BP26,进行DNA测序后,与羊标准菌株比对分析。结果 9株致病菌株均为羊种布鲁氏菌,与标准菌株相应基因序列比对发现,9株布鲁氏菌与标准菌株间存在相同或不同水平的碱基差异。碱基突变、缺失、插入等形式均有出现。结论证实从患者血液中分离得到的菌株均为羊种布鲁氏菌,并与标准菌株间存在分子差异。

Objective To isolate 9 strains of pathogens from the blood of patients and identify the species of the strains,and to analyze the differences between the nine bacterias and the standard strains on DNA levels. Methods Conventional type test were carried out to identify that all of the 9 stains were the Brucella. Molecular biology techniques were used to clone the pathogenic genes OMP25,OMP31,UGPB and BP26 of the nine Brucella, DNA sequencing and comparative analysis. After sequnecing, com-pared with the standard strain. Results All of the 9 strains of pathogens were Brucella melitensis,and compared with the corre-sponding gene sequences of standard strains. We found that there were the same or different differences between the nine strains and the standard strain on DNA levels. Base mutations, deletions,insertions, and other forms have appeared. Conclusion It could be confirmed that the 9 strains isolated from the blood of the patients were Brucella melitensis,and had the molecul＆r differences be-tween the 9 stains and the standard strain.