H_2O_2溶液对2种红葡萄离体叶中主要茋化物含量的影响

The Impact of H_2O_2 Treatment on Stilbene Contents in the Detached Leaves

比较H2O2诱导后美国红提(‘红地球’)和紫香无核(‘新农4号’)离体葡萄叶片中的白藜芦醇(Res)、白藜芦醇苷(PD)、紫檀茋(PS)、ε-二聚体葡萄素(ε-VF)4种茋化物的含量,为利用葡萄生产废弃叶片的综合利用提供依据。采用H2O2溶液对红提和紫香无核离体葡萄叶片进行诱导,采用高效液相色谱法分析4种茋化物的含量变化。H2O2诱导后的两种离体叶片4种茋化物的积累呈现显著的量时依赖性;2.5%H2O2处理48h后叶片4种茋化物含量可提高6倍以上,以红提的含量相对较高,总茋化物鲜重达到138.82μg/g。用H2O2诱导后的离体葡萄叶中4种茋化物含量比诱导前有了较为明显的增加。

The aims were to compare the contents of resveratrol（Res）,piceide（PD）,pterostilbene（PS）,ε-viniferin（ε-VF） in the grape leaves of Hongti（＇Vitis vinifera cv.Red globe＇） and Zixiangwuhe（＇ Vitis vinifera cv.NO.4 Xin nong＇） treated with H2O2,and provide the basis for the comprehensive utilization of waste grape leaves.In present study,the detached grape leaves Hongti and Zixiangwuhe were treated with H2O2 solution to investigate the changes of stilbenes contents by HPLC methods.The results showed those stilbenes accumulated significantly in both dose-dependent and time-dependent manners in H2O2-treated leaves.4 kinds of stilbenes increased 6 times higher than untreated,with 2.5% H2O2 for 48 hours treatment.Stilbenes contents of Hongti grape leaves was relatively high,the maximum contents of the total stilbenes compounds was 138.82 μg/g in treated leaves.H2O2-treated detached grape leaves showed notably increase on stilbenes contents than blank.