摘要
研究碳离子注入及注入次数对不同品种向日葵生长发育和遗传变异的影响。分别向‘巴葵138’、‘巴葵136’、‘巴葵118’种子注入5×1013C/cm2碳离子,种植后采收种子进行2次辐照。继续田间试验,观察记录不同巴葵的生长状况,采用RAPD进行遗传变异分析。‘巴葵138’一次辐照组种子发芽率及植株株高、茎粗、叶片数、产量等多项性状都显著提高,二次辐照组则相反;‘巴葵136’和‘巴葵118’变异不显著。分子水平上,一次和二次辐照组与对照组的遗传距离在‘巴葵138’中依次为0.1111、0.1087,‘巴葵136’中为0.0680、0.0303,‘巴葵118’为0.0625、0.0435。碳离子注入对‘巴葵138’的生长发育有显著影响,其效应因辐照次数而异,同时能引起3个品种向日葵基因组DNA产生变异。
The aim was to study the growth and genetic variation effects of carbon ion implantation and implantation times on different kind of sunflowers.Carbon ions were implanted into ＇ Ba-sunflower138 ＇,＇ Ba-sunflower136 ＇ and ＇ Ba-sunflower118 ＇ seeds in dose of 5 × 10 13 C/cm 2 respectively,planting and harvesting the seeds to get irradiated again.The author continued the field test,observed the growth conditions of different Ba-sunflowers,and analyzed the genetic variation with RAPD.The germination rate and a number of traits like plant height,stem diameter,leaf number and yields of ＇ Ba-sunflower138 ＇ had a significant improve,while the twice-irradiated group just the opposite;the variation of ＇ Ba-sunflower136 ＇ and ＇ Ba-sunflower118 ＇ was not significant.At the molecular level,the genetic distance with the control group of once and twice-irradiated groups was 0.1111,0.1087 in ＇ Ba-sunflower 138 ＇ ;0.0680,0.0303 in ＇ Ba-sunflower136 ＇ and 0.0625,0.0435 in ＇ Ba-sunflower118 ＇.Carbon ion implantation had a significant effect on the growth and development of ＇ Ba-sunflower138 ＇,and its effect varies with irradiation times,at the same time,it could cause genomic variation in the three kinds of sunflowers.
出处
《中国农学通报》
CSCD
2013年第6期175-181,共7页
Chinese Agricultural Science Bulletin
基金
射线束技术与材料改性教育部重点实验室基金课题"辐射处理植物花粉和营养器官诱变效应的研究"(201123)
