水貂肠道酵母菌的分离鉴定及系统发育分析

Isolation and Identification and Phylogenetic Analysis of Yeast Strains from the Intestinal Tract of Mink

为了获得水貂专用的有益菌种,以为研究毛皮动物益生菌制剂提供理论基础。从健康水貂的肠道中分离纯化出2株酵母菌,编号为JM1和JM2,进行了形态学观察、生理生化试验以及26SrDNA基因序列分析,并构建了系统发育进化树,同时对其生长情况和安全性等进行了综合鉴定。传统鉴定方法和26SrDNAD1/D2区序列分析结果表明:JM1为弗比恩毕赤酵母,菌株JM2为酿酒酵母;生长情况结果表明:2菌株在14～30h生长较快,在30～40h生长趋于平稳;2菌株培养液中糖度12h时波美度开始急速下降,并且在26h后有升高趋势;安全性试验显示:2菌株对小鼠均无毒性,且在2.73×107cfu/mL时小鼠生长速度最快。

In order to get the mink dedicated beneficial bacteria and provide a theoretical basis for the study fur animal probiotic preparations.Two strains of yeasts were isolated and purified from the intestines of healthy mink named JM1 and JM2.Morphological observation,physiological and biochemical tests and 26S rDNA gene sequence analysis,and a phylogenetic tree were constructed.Meanwhile,these two bacteria ' s growth and security were comprehensive identification.The traditional identification methods and 26SrDNA D1/D2 sequence analysis results were showed that:JM1 was identified as Saccharomyces cerevisiae strain and JM2 was Pichia fabianii strain;The growth result was that:the growth speed of two strains were faster in 14-30 h and the growth speed were stabilized in 30-40 h;sugar content in the culture medium of the two strains Baume degrees began to rapidly decline at 12 h,and showed an increasing trend after 26 h.Safety test results were showed that:2 strain had no toxicity on mice,and the mice grew at the fastest speed at the 2.73 × 10 7 cfu/mL dose.