中华鳖太湖种群淋巴细胞培养及染色体核型分析

The Lymphocytic Cell Culture and Chromosome Karyotype of Trionyx sinensis in Taihu Lake Population

为给中华鳖种质研究和染色体操作提供理论依据。以中华鳖太湖种群为材料,采用淋巴细胞培养、空气干燥法制片、Giemsa染色,对中华鳖染色体核型进行了分析。结果表明:通过淋巴细胞培养法可以得到良好的染色体制片;中华鳖太湖种群具有33对染色体,其中6对是大型染色体,27对是小型染色体。

In order to provide the logical proofs for germplasm research and chromosomal manipulation of Trionyx sinensis.The karyotypes of the chromosomes of Trionyx sinensis in Taihu Lake were examined by lymphocytic cell cultivation-air drying-Giemsa staining method.The results showed that the chromosome karyotype of T.sinensis in Taihu Lake population could be obtained through the lymphocytic cell cultivation method.There were 33 pairs chromosome including 6 pairs of large chromosome and 27 pairs of smaller chromosome.