水生植物热解生物油对中肋骨条藻抗氧化酶活性的影响

Effects of Macrophytes Pyrolysis Bio-oil on Skeletonema costatum Antioxidant Enzyme Activities

为揭示水生植物生物油抑藻机制,研究了芦竹300℃、芦苇400℃以及香蒲400℃这3种生物油对中肋骨条藻丙二醛含量变化及抗氧化酶系统(SOD、POD、CAT)活性的影响.结果表明,在这3种生物油作用下,生物油浓度越高MDA含量越高,当生物油浓度为10 mg·L-1时,中肋骨条藻丙二醛(MDA)含量随着作用时间的延长而升高;超氧化物歧化酶(SOD)活性随生物油添加浓度的升高而升高,在芦竹300℃及香蒲400℃生物油作用下随时间延长呈先升高后降低趋势,均在24 h时达到最大值,分别为93.6 U.(107cells)-1、8.23 U.(107cells)-1,而在芦苇400℃生物油作用下72 h内始终保持升高趋势;过氧化物酶(POD)活性同样也随生物油浓度的增加而升高,芦竹300℃、芦苇400℃生物油作用下POD活性先升后降,香蒲400℃作用下则呈波动上升趋势;过氧化氢酶(CAT)活性在这3种生物油作用下随培养时间的延长均呈现先升高后降低的趋势,且生物油浓度越大,CAT活性越高.生物油引起抗氧化酶活性升高,导致藻细胞内产生氧化胁迫,可能是其抑制藻类的主要机制.

In order to reveal the preliminary inhibition mechanisms of aquatic plants bio-oils on Skeletonema costatum, effects of Arundo donax L. 300℃ , Ph. australis Trin. 400℃ and Typha orientalis Presl 400℃ bio-oils on the concentration change of malondialdehyde （MDA） and the activity of antioxidant enzymes system （SOD, POD and CAT） were evaluated. The results showed that the higher the Bio-oil concentrations, the higher the MDA contents in Skeletonema costatum was, and when the Bio-oil concentration was 10 mg.L-1 , the MDA concentration increased with the reaction time. Superoxide dismutase （SOD） activity also increased with the increase of bio- oil concentration. For Arundo donax L 300℃ and Typha orientalis Presl 400℃ bio-oil, when the reaction time was longer, the SOD activity of Skeletonema costatum first increased and then decreased, and in both cases the maximum SOD activity was measured at 24 h, reaching 93.6 U. （ 107 cells） -1 and 8.23 U. （ 10^7 cells） -1, respectively. For Ph. australis Trin 400℃ bio-oil, the SOD activity kept increasing within 72 h. The peroxidase （POD） activity of Skeletonema costatum also increased with the increase of bio-oil concentrations. In the presence of Arundo donax L. 300℃ and Ph. australis Trin 400℃ bio-oil, the POD activity of Skeletonema costatum first increased and then decreased, while with Typha orientalis Presl 400℃ bio-oil the POD activity increased with fluctuations. For all the three bio-oils, the catalase （CAT） activities increased first and then decreased when the reaction time was prolonged, and the higher the bio-oils concentration, the greater the CAT activity was. Pyrolysis bio-oils enhance the activity of antioxidant enzymes, leading to intracellular oxidative stress in the algae, which seems to be the main inhibitory mechanism for algae.