D-试验对葡萄球菌对克林霉素诱导型耐药的检测分析

Detection for inducible clindamycin resistance in staphylococcus by D-test

目的分析葡萄球菌对红霉素诱导克林霉素耐药性的发生率及其耐药表型。方法用K-B法检测葡萄球菌属对红霉素及克林霉素的体外敏感性,依据CLSI2011年推荐的D-试验(即克林霉素诱导耐药实验)测定红霉素对克林霉素的诱导耐药表型。结果红霉素和克林霉素同时耐药菌株占所测菌株的61.2%,D-实验阳性菌株占所测菌株的11.9%,占红霉素耐药克林敏感菌株的56.5%。结论 D-试验的阳性发生率较高,微生物实验室应常规进行诱导型耐药检测以指导临床合理使用抗生素。

Objective To analyze the prevalence and phenotypes of inducible clindamycin resistance of staphylococcus in our hospital. Methods The susceptibility to erythromycin and clindamycin for staphylococcus was examined by Kirby-Bauer disc agar diffusion test and the inducible resistance of erythromycin to clindamycin was checked by D-test according to the stand- ards of CLSI 2011. Results Co-resistance strains to erythromycin and clindamycin accounted for 61.2%. D-test positive rate was 11.9% among all staphylococcus tested, and it was 56.5% among the erythromycin-resistant and clindamycin-sensitive staphylococcus. Conclusion A high rate of positive D-test results is observed in the test. The detection of inducible ctindamycin resistance should be conducted routinely in microbiological laboratory to guide appropriate use of antibiotics in clinical practices.