一氧化氮抑制甘氨鹅脱氧胆酸诱导A549细胞凋亡的实验研究

Experimental study on inhibition of NO in process of Glycochenodeoxycholate inducing A549 cell apoptosis

目的探讨一氧化氮对甘氨鹅脱氧胆酸诱导A549细胞凋亡的抑制作用及其机制。方法成功建立胆甘氨鹅脱氧酸诱导A549细胞凋亡模型后,检测一氧化氮合酶的表达水平及一氧化氮供体对甘氨鹅脱氧胆酸诱导A549细胞凋亡的抑制作用。结果甘氨鹅脱氧胆酸可诱导A549细胞发生caspase-3依赖的细胞凋亡,凋亡过程中伴随有一氧化氮合酶表达抑制,一氧化氮供体能有效地减弱胆酸诱导的A549细胞凋亡。结论通过吸入一氧化氮可有效抑制甘氨鹅脱氧胆酸诱导肺泡Ⅱ型上皮细胞凋亡。

Objective To explore the inhibitory effect of nitric oxide （NO） on Glycochenodeoxycholate （GCDC） induced A549 cell apoptosis and its mechanism. Methods After establishing apoptosis model of A549 cell which was induced by GCDC,the expression of inducible nitric synthase （iNOS） and ＇inhibitory effect of NO donors on inducing A549 cell apoptosis by GCDC were detected. Results GCDC can induce A549 cell apoptosis relied on caspase3. The expression of iNOS was inhibited during the process of apoptosis,and the donors of NO can reduce A549 cell apoptosis effectively. Conclusion NO inhalation can effectively inhibit the process of alveolar epithelial cell Ⅱ （AECII） apovtosis induced bv GCDC.