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抑癌基因maspin在胃癌细胞株SGC-7901中的表达及意义 被引量：1

Expression and significance of maspin gene in gastric cancer cells SGC-7901
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摘要 目的探讨抑癌基因maspin在胃癌细胞株SGC-7901生长和凋亡过程中的生物学作用。方法构建重组质粒pCD-NA3.1-maspin,转染胃癌细胞株SGC-7901,采用逆转录-聚合酶链反应(RT-PCR)和免疫细胞化学检测maspin基因表达,MTT法检测细胞增殖,原位末端标记法(TUNEL)检测细胞凋亡。结果重组质粒pCDNA3.1-maspin导入SGC-7901细胞株后,maspin的mRNA和蛋白表达明显增强(P<0.05),胃癌细胞株SGC-7901增殖受到抑制,并出现明显的细胞凋亡。结论 maspin基因可以抑制胃癌SGC-7901细胞增殖,并诱导细胞凋亡。 Objective To investigate the biological effect of maspin gene on growth and apoptosis of gastric carcinoma cell SGC- 7901 in vitro. Methods pCDNA3.1-maspin vector was constructed and transfected into SGC-7901 cells. Maspin gene expression was detected with RT-PCR and cell immunochemistry. Cells growth rate was detected with MTT,and cell apoptosis was tested by TUNEL. Results After transfected with pCDNA3. 1-maspin, both RNA and protein levels of maspin increased obviously（P（ 0. 05）. The proliferation of gastric cancer cells SGC-7901 was suppressed obviously, and cell apoptosis is obvious. Conclusion Maspin gene can suppress the proliferation of gastric carcinoma ceils arid induce cell apoptosis.
作者 谯敏 牛司强 王丕龙
机构地区 重庆医科大学附属第一医院消化内科 重庆医科大学附属第一医院检验科
出处 《重庆医学》 CAS CSCD 北大核心 2013年第6期604-606,615,共4页 Chongqing medicine
基金 重庆市卫生局资助项目(2008-2-121) 重庆医科大学校办资助项目(XBYB2007021)
关键词 胃肿瘤 细胞增殖 细胞凋亡 MASPIN stomach neoplasms cell proliferation apoptosis maspin
分类号 R735.2 [医药卫生—肿瘤]
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参考文献15

  • 1Chan AD, Wong BC, Lam SK. Gastric cancer: past, pres ent and future[J]. Can Gastroenterol J, 2001,15 ( 7 ) : 469-474.
  • 2Shi HY, Zhang W, Liang R, et al. Modeling human breast cancer metastasis in mice, maspin as a paradigm[J]. His- tol Histopathol, 2003,18( 1 ) : 201-206.
  • 3Casado E, Comez-Navarro J, Yamamoto M, et al. Strate gies to accomplish targeted expression of transgenes in o varian cancer for molecular therapeutic appliacations[J] Clin Cancer Res,2001,7(8) :2496-2504.
  • 4Zou Z, Anisowicz A, Hendix MJC, et al. Maspin, a serine with tumor suppressing activity in human mammary epi- thelial cells[J]. Science, 1994,263 ( 10 ) : 526-529.
  • 5Zou Z, Cao C, Nagaieh AK, et al. p53 regulates the ex- pression on of the tumor suppressor gene Maspin[J]. J Biol Chen,2000,275(9) :6051-6054.
  • 6Abraham S, Zhang W, Greenberg N, et al. Maspin func- tions as tumor suppressor by increasing cell adhesion to extracellular matrix in prostate tumor cells [J]. J Urol, 2003,169(3) :1157-1161.
  • 7Shao LJ, Shi HY, Ayala G, et al. Sufficiency of the maspin tumor suppressor gene leads to hyperplastic lesions in prostate [J]. Cancer Res,2008,68(13) :5143-5150.
  • 8Lonardo F,Li X,Kaplun A,et al. The natural tumor sup- pressor protein maspin and potential application in non small cell lung cancerEJ~. Curr Pharm Des,2010,16(16) 1877-1880.
  • 9Mohsin SK, Zhang M, Clark GM, et al. Maspin expression in invasive breast cancer., association with other prognos- tic factors[J]. J Pathol,2003,199(4) :432-435.
  • 10Marioni G,Giacomelli L, Alessandro E, et al. Nuclear lo- calization of mammary serine protease inhibitor (MASPIN):is its impact on the prognosis in laryngeal carcinoma due to a pro-apoptotic effect[J]. Am J Otolar- yngol, 2008,29(3) : 156-162.

同被引文献15

  • 1Zou Zv Anisowicz A.Hendrix MJ.et al. Maspinv a serpin with tumor-suppressing activity in human mammary epi?thelial cells[J]. Science.1994.263(5146) :526-529.
  • 2Zou Z. Cao C. Nagaich AK. et al. p53 regulates the ex?pression on of the tumor suppressor gene mas pin[J].J Bioi Chen.2000.275(9) :6051-6054.
  • 3Abraham S. Zhang W. Greenberg N. et al, Maspin func?tions as tumor suppressor by increasing cell adhesion to extracellular matrix in prostate tumor cells[J].J Urol , 2003.169(3):1157-1161.
  • 4KimJ .Jang KT .Kim KH.et al. Aberrant mas pin expres?sion is involved in early carcinogenesis of gallbladder cancer[J]. Tumour Biol.2010.31(5) :471-476.
  • 5Nam EvPark C. Maspin suppresses survival of lung cancer cells through modulation of Akt pathway[J]. Cancer Res Treat.2010.420) :42-47.
  • 6Tsuji T. Umekita Y. Ohi Y. et al. Maspin expression is up-regulated during the progression of endometrioid en?dometrial carcinoma[J]. Histopathology. 2007.51 (6) : 871-874.
  • 7Sood AK.Fletcher MS. Gruman LM.et al. The paradoxi?cal expression of mas pin in ovarian carcinoma[J]. Clin Cancer Res.2002.8(9) :2924-2932.
  • 8Mohsin SK.Zhang M.Clark M.et al. Maspin expression in invasive breast cancer: association with other prognos?tic factor[J].J Pathol.2003.199(4) :432-435.
  • 9Lonardo F.Li Xv Kaplun Avet al. The natural tumor sup?pressor protein mas pin and potential application in non small cell lung cancer[J]. Curr Pharrn Des. 2010. 16 (6): 1877.
  • 10Liu Zv Shi H'YvNawaz Z.et al. Tamoxifen induces the ex?pression of mas pin through estogen receptor-alpha[J]. Cancer Lett.2004.2090) :55-65.

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重庆医学
2013年 第6期

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