荧光实时定量PCR对肺癌相关基因检测及其临床意义

Application of real-time fluorescent quantitative PCR in detection of lung cancer-related genes and its clinical significance

目的采用荧光实时定量PCR对肺癌相关基因检测并建立辅助诊断指数。方法采用荧光实时定量PCR对肺癌患者手术标本LUNX、CK19、CEA、MUC1及Survivin等肺癌相关基因mRNA进行检测,运用2-△△CT算法计算相对表达量,并以此建立辅助诊断指数。结果 LUNX、CK19、CEA、MUC1及Survivin在正常肺组织、手术切缘、癌旁与肿瘤组织间的表达比较差异有统计学意义(P<0.05),且呈现递增趋势;设定辅助诊断指数大于或等于3.5为肺癌时,其敏感度95%,特异度为92%,阳性结果预示值(PPV)为95.33%,阴性结果预示值(NPV)为98.00%。结论利用荧光实时定量PCR对肺癌相关基因进行检测并建立辅助诊断指数,能有助提高肺癌诊断的敏感度与特异度。

Objective To apply real-time fluorescent quantitative PCR to detect lung cancerrelated genes and established molecular diagnostic index. Methods Applied real-time fluorescent quantitative PCR to detect gene LUNX, CK19,CEA, MUC1 and Survivin of surgical specimen from patients with lung cancer. Relative expression of these genes were calculated by 2-^△△CT method, based on which,MDI was established. Results Differen.tial expression of LUNX, CK19,CEA, MUC1 and Survivin were found in normal,surgical margin, paratumor and tumor tissues（P〈0.05） ,a significant decline trend was found. When MDI was set ≥3.5 e- quivalent to cancer, MID＇s sensitivity was 95 %, specificity was 92 %, positive result predicted value （PPV） was 95.33 %, negative results predicted values （NPV） was 98.00 %. Conclusion Sensitivity and specificity of diagnosis of lung cancer could be enhanced by MDI which is established based on real-time fluorescent quantitative PCR detection of related genes.