吲哚青绿介导光动力疗法治疗兔眼后发性白内障中视网膜的毒性观察

Observation on the toxic effect of indocyanine green mediated photodynamic therapy on retina of rabbits with after-cataract

目的研究吲哚青绿介导光动力疗法治疗兔眼后发性白内障后,兔眼视网膜的损伤情况。方法 32只兔眼随机分为对照组、低剂量组、中剂量组、高剂量组,分别行晶状体超声乳化及皮质吸出术,低、中、高剂量组术后前房内注入0.5mL浓度分别为1.25、2.50、5.00mg/mL的吲哚青绿,用810nm的半导体激光对囊膜进行低能量(50mW/cm)照射2min。术后3个月时对兔眼进行闪光视觉诱发电位检查后取材行透射电镜观察兔眼视网膜有无损伤。结果晶状体后囊膜混浊情况均有减轻,各组间比较差异有统计学意义(P<0.01)。同时前房内注入浓度为1.25mg/mL吲哚青绿组兔眼视网膜电镜损伤相对较小,各实验组行闪光视觉诱发电位结果之间差异无统计学意义(P>0.05)。结论吲哚青绿介导的光动力疗法可明显降低术后晶状体后囊膜混浊的程度,而同时吲哚青绿浓度为1.25mg/mL治疗组对视网膜的损伤最小。

Objective To observe the toxic effect of indocyanine green mediated photodynamic therapy on retina in rabbits with after-cataract. Methods 32 rabbits＇ eyes were randomly divided in 4 groups. Extracapsular lens extraction by phacoemulsification was operated on rabbits＇ eyes. 0.5 mL ICG （1.25 mg/mL,2.50 mg/mL,and 5.00 mg/mL） was injected into the anterior chamber after surgery respectively,and the eye was illuminated with a diode laser （wavelength 810 nm） for 2 rain. 3 months after surgery, the toxic effect on retina was observed by electron microscope and FVEP. Results Each group had effects and degree of the rabbits＇ PCO was related to the dose of ICG（P〈0.01）. The toxic effect on retina in 1.25 mg/mL group was less than that in other groups. The result of FVEP in each group had no significant difference （P〉0.05）. Conclusion PDT with ICG greatly reduces the degree of PCO. The toxic effect on retina in ICG of 1.25 mg/mL group was least.