6-羟基山萘酚-3-O-β-葡萄糖苷抑制脱氧核糖氧化损伤的机理

Mechanism Underlying Protective Effect of 6-Hydroxykaempferol-3-O-β-glucoside Deoxyribose against Oxidative Damage to Deoxyribose

目的:研究6-羟基山萘酚-3-O-β-葡萄糖苷(6-HK-3-O-G)抑制Fenton反应和对羟自由基清除的能力,并对其抑制Fenton反应的机理进行初步探讨。方法:利用脱氧核糖氧化损伤的实验检测模型和DPPH自由基的清除实验。结果:6-HK-3-O-G对羟自由基具有较强的清除能力;对Fenton反应引起的2-脱氧核糖氧化损伤具有保护作用,其保护作用机理与6-HK-3-O-G直接对Fenton反应所产生的羟自由基的清除作用有关,但更主要的可能是由于6-HK-3-O-G与Fe2+的络合作用,阻断了Fenton反应的进行。结论:6-HK-3-O-G具有强的抗氧化活性,在生理条件下,6-HK-3-O-G与Fe2+的络合作用可能是其抑制Fenton反应的关键因素。

Objective： To evaluate the capability of 6-hydroxykaempferol-3-O-β-glucoside （6-HK-3-O-G） for inhibiting Fenton reaction and scavenging hydroxyl flee radicals, and to explore the inhibitory mechanism on Fenton reaction. Methods： A model to test oxidative damage to deoxyribose and a reaction system to test DPPH free radical scavenging activity were created. Results： 6-HK-3-O-G not only had strong scavenging activity against hydroxyl free radical, but also could protect 2-deoxyribose against oxidative damage caused by Fenton reaction. The underlying mechanisms were related to the direct scavenging of hydroxyl free radical generated from Fenton reaction but seemed to be mainly due to the chelation of 6-HK-3-O-G with Fe2＋, thereby blocking Fenton reaction. Conclusion： 6-HK-3-O-G has strong anti-oxidation activity, and the chelation of 6-HK-3-O-G with Fe2＋ may play a vital role for its inhibiting Fenton chemistry under physiological conditions.