微波协同辅助复合酶法制备高F值玉米肽条件的优化

Optimization of Preparation Process of High F-Value Corn Peptide by Microwave-Assisted Enzymatic Hydrolysis

对微波协同辅助复合酶法制取高F值玉米肽的条件进行优化。以超临界CO2脱脂除杂处理后的玉米蛋白粉(CGM)为原料,采用Alcalase 2.4L与木瓜蛋白酶进行分步水解。通过单因素与正交试验,确定微波协同酶解法制取高F值玉米肽的最佳条件。结果表明:Alcalase 2.4L蛋白酶最佳酶解条件为底物含量9g/100g、酶添加量([E]/[S])3g/100g、pH9.0、微波功率300W、微波时间2.5min,此条件下多肽得率为23.80g/100g CGM;木瓜蛋白酶酶解的最佳条件为酶添加量([E]/[S])4g/100g、pH7.5、微波功率100W、微波时间5min,此条件下耗碱量为6.22mL/100mL。使用微波协同酶法所制取的玉米肽F值为27.15,比传统的水浴酶法提高了21%,且极大地缩短了反应时间。

Microwave-assisted enzymatic hydrolysis conditions for preparing high F-value corn peptide were optimized. Corn gluten meal （CGM） was used as the raw material and degreased by supercritical CO2 fluid. Alcalase 2.4L and papain were used to hydrolyze CGM. The optimal alcalase hydrolysis conditions were substrate concentration of 9 g/100 g, alcalase- substrate ratio of 3 g/100 g, hydrolysis pH of 9.0, microwave power of 300 W and microwave treatment time of 2.5 min. Under the optimal conditions for alcalase, the yield of corn peptide was up to 23.8 g for 100 g CGM. Similarly, the optimal papain hydrolysis conditions were papain-substrate ratio of 4 g/100 g, hydrolysis pH of 7.5, microwave power of 100 W, microwave treatment time of 5.0 rain and alkali consumption of 6.22 mL/100 mL. The F value of the corn peptide prepared by microwave-assisted enzymatic hydrolysis was 27.15, which revealed an improvement by 21% when compared with traditional water-bath enzymatic hydrolysis. Moreover, microwave-assisted enzymatic hydrolysis time was shortened.