屎肠球菌冷冻干燥保护剂筛选及稳定性比较研究

Selection and Stability of Cryoprotectants for Enterococcus faecium during Freeze-Drying

屎肠球菌在微生态产品中的应用效果受产品活菌量、贮存稳定性影响明显,为寻找适于屎肠球菌规模化生产的冻干保护剂,本研究通过正交试验设计,及屎肠球菌冷冻干燥保护剂4℃、37℃贮存稳定性比较,优化适合屎肠球菌的冻干保护剂配方为:脱脂乳5g/100mL、蔗糖1g/100mL、麦芽糊精5g/100mL。采用此保护剂配方制备的屎肠球菌冻干样品,在铝箔袋密封包装、温度37℃、相对湿度75%条件下存放4周,菌存活率为97.90%。

Enterococcusfaecium is one strain of normal microflora of human and animal intestinal tracts. The bacterial colony has regulative function to intestines. The application of Enterococcus faecium in probiotics is highly limited due to the number of vial bacteria and storage stability. In this study, the optimal cryoprotectant formula for Enterococcusfaecium was explored using orthogonal array design and storage stability was tested at 4℃ and 37 ℃. The optimal cryoprotectant formula was composed of 5 g/100 mL skim milk, 1 g/100 mL sucrose and 5 g/100 mL maltodextrin. The survival rate of Enterococcusfaecium using this optimal formula in sealed aluminum foil bag with temperature of 37 ℃ and relative humidity of 75% was up to 97.90% after the storage for 4 weeks.