植物乳杆菌NDC 75017的降胆固醇作用

Cholesterol-Lowering Capability of Probiotic Lactobacillus plantarum NDC75017

植物乳杆菌NDC 75017分离自中国内蒙古传统发酵乳制品。本实验对其体外环境耐受性及其降胆固醇作用进行研究。采用活菌计数法检测植物乳杆菌NDC 75017在pH 2.0～3.0的酸性环境、胆盐质量浓度0.3g/100mL、NaCl质量浓度0～7g/100mL以及模拟人工消化液环境下的耐受情况;同时以邻苯二甲醛法测定其体外降胆固醇能力。结果表明:植物乳杆菌NDC 75017可耐受pH3.0及0.3g/100mL胆盐环境;在7g/100mL的高盐质量浓度条件活菌数仍可达108CFU/mL;可以通过胃进入肠道而保持活性,在肠道处理8h后存活率为58.73%。体外降胆固醇实验测得细菌发酵上清液、菌体洗涤液和细胞破碎液中的胆固醇脱除率分别为16.43%、26.35%和32.87%。植物乳杆菌NDC 75017环境耐受能力良好,具有体外降胆固醇作用,该菌株可以作为降胆固醇的潜在益生菌。

Lactobacillus plantarum NDC 75017 was isolated from traditional dairy products in Inner Mongolia of China. The aim of this study is to evaluate the environment-tolerant capability and cholesterol-lowering effect of L. plantarum NDC75017. Viable count was firstly used in this study to detect the tolerance characteristics under simulated gastrointestinal tract environment pH 2.0--3.0. Additionally, bacterial growth was evaluated in the presence of 0.3 g/100 mL bile salt and 0--7 g/100 mL sodium chloride. O-phthalaldehyde was used to detect its cholesterol-lowering capability. The results showed that L. plantarum NDC75017 had tolerance characteristics against pH 3.0 and 0.3 g/100 mL bile salt. The viable bacteria can be up to 108 CFU/mL at sodium chloride of 7 g/100 mL. L. plantarum NDC75017 kept vitality from stomach to intestines. After 8 h incubation in artificial intestinal fluid, the survival rate of the strain remained up to 58.73%. Its cholesterol-lowering rates in bacterial fermentation supernatant, bacteria washing solution and cell disruption solution were 16.43%, 26.35% and 32.87%, respectively. These results indicated that L. plantarum NDC75017 had environment-tolerant characteristics and could be as the probiotics with cholesterol-lowering activity.