黑米花青苷复方胶囊对实验性肝损伤的保护作用

Protective Effect of Black Rice Anthocyanin Compound Capsule against Experimental Hepatic Injury

建立小鼠四氯化碳(CCl4)肝损伤病理模型,分别灌胃低剂量(7.57mg/(kg.d))、高剂量(10.60mg/(kg.d))的黑米花青苷复方胶囊进行治疗,正常组灌胃蒸馏水,助悬剂组灌胃大豆油。5周后,测定肝组织中超氧化物歧化酶(SOD)活力、丙二醛(MDA)含量、谷胱甘肽(GSH)含量及肿瘤坏死因子-α(TNF-α)水平,并进行肝脏病理学观察。结果表明:黑米花青苷复方胶囊能明显提高肝组织中SOD的活力,对MDA起到直接清除作用;能提高GSH含量,降低TNF-α的水平;同时,能有效显著改善肝组织的纤维化。助悬剂对药物的效果基本没有影响。因此,黑米花青苷复方胶囊能够对CCl4肝损伤起到一定的防治作用。

Pathological model of carbon tetrachloride （CCl4）-induced hepatic injury in mice was established for the treatment by black rice anthocyanin compound capsule at low and high doses through gavage. The administration of distilled water was used as the normal group, and plant oil was used as the suspending agent. The contents of SOD, MDA and GSH as well as the level of TNF-α in liver homogenate were measured after 5 weeks. Hepatic pathological examination was also conducted. The results showed that black rice anthocyanin compound capsule could increase SOD activity obviously, clear MDA content directly, improve GSH content and reduce TNF-α level in hepatic tissue. The pathological analysis suggested that black rice anthocyanin compound capsule improved hepatic fibrosis in mice. Suspending agent did not exhibit an obvious influence on drug efficacy. Therefore, black rice anthocyanin compound capsule has a particular treatment effect on CC14-induced hepatic injury.