沙樱桃脂类对人MGC-803细胞抑制作用的实验研究

Inhibition effects of shayingtao lipids on the human gastric carcinoma MGC-803 cells

目的探讨沙樱桃脂类对人胃癌MGC-803细胞的毒性作用及其机制。方法采用台盼蓝法检测细胞数、流式细胞仪检测细胞凋亡。将人MGC-803细胞分别与不同浓度的沙樱桃脂类(沙樱桃脂组)、5-FU(5-FU组)及PBS溶液(对照组)共同培养,观察沙樱桃脂类对人胃癌MGC-803细胞增殖的抑制作用。结果对人MGC-803细胞分别给予0.5、1.0、3.0mg/L沙樱桃脂类及10mg/L5-FU,培养6h、12h、18h、24h和48h,活细胞数随沙樱桃脂类浓度的增加和作用时间的延长而减少,与5-FU组相似(P>0.05),而与对照组比,差异有统计学意义(P<0.01);分析存活细胞率,表明人MGC-803细胞增殖被阻滞于G0G1期。在给予沙樱桃脂类0.5、1.0、3.0mg/L和5-FU培养48h后,人MGC-803细胞的凋亡率分别为13.8%、19.6%、26.2%和21.1%。结论沙樱桃脂类对人胃癌MGC-803细胞具有显著毒性作用,其机制可能与沙樱桃脂类可抑制MGC-803细胞分裂,并诱导其凋亡有关。

Objective To investigate toxic effect of shayingtao lipids on human gastric carcinoma MGC-803 cells proliferation and its potential mechanism. Methods The living cell were determined by trypan blue method, and the cell apoptosis was detected by flow cytometry. The MGC-803 cells were cultured together with shayingtao hpids of different concentration （ shayingtao hpids group）, 5-FU （ 5-FU group） and PBS solution （ control group） respectively. The inhibition effects of shayingtao lipids and 5-FU on MGC-803 cell proliferation were observed and compared. Results Culturing MGC-803 ceils together with 0.5,1.0,3.0 nag / L shayingtao lipids and 10mg/L 5FU for 6h, 12h, 18h, 24h and 48h, the number of living cells decreased along with the increase of shayingtao lipids concentration and prolongation of action time, and the result was similar with 5-FU （ P 〉 O. 05 ） while had statistical difference compared with control group （P 〈 O. 01 ）. According to analysis of cell living rate, the proliferation of MGC-803 cells was blocked in GoGl stage. Culturing MGC-803 cells together with O. 5,1. O, 3.0 mg/L shayingtao lipids and 10 mg / L 5-FU for 48h,the apoptosis rates of MGC-803 cell were 13.8%, 19.6% ,26.2% and 21.1% respectively. Conclusion Shayingtao lipids have significant toxic effect on human gastric carcinoma MGC-803 cells, and the mechanism might be implicated in inhibiting cell division and inducing cell apoptosis.