摘要
研究了地毯草黄单胞菌(Xanthomonas axonopodis)ABB-2对红掌品种"阿拉巴马"的致病性及其防御相关生化影响.结果表明,红掌对该病菌高度敏感,离体叶片接种第4天、盆栽植株接种第8天后,病叶率达到100%.体叶内超氧化物歧化酶(SOD)、过氧化物酶(POD)、多酚氧化酶(PPO)和苯丙氨酸解氨酶(PAL)活性增加,分别在接种后的第4、6、8和12天达到峰值,较非接种分别对照增加了15.37%、35.63%、51.89%和66.43%;总可溶性蛋白、游离酚含量最大值分别为非接种对照的121.76%和134.66%.
The disease development and defense- related biochemical changes in the leaves of Anthurium andrae- hum cultivar ' Alabamb' were assayed after inoculation with Xanthomonas axonopodis ABB - 2. Pathogenicity assay revealed that' Alabamb' was highly sensitive to X. axonopodis. On the day 4 in infection experiment of detached leaf and on the day 8 in infection experiment of pot plant with the X. axonopodis, all inoculated leaves developed disease symptoms of bacterial blight, and the percentage of leaves with lesions reached 100%. The activities of four tested defense- related enzymes, SOD, POD, PPO, and PAL in the leaves of Anthurium ' Alabamb' increased and reached the peaks on days 4, 6, 8 and 12 after inoculation with X. axonopodis ABB - 2, with the increase values of 15.37%, 36.62%, 46. 68%, and 66.43%, respectively, when compared to those of non- inoculated control plant. After the peaks, SOD and POD activities had a continuous decrease until the level of lower than that of the non - inoculated control plant. And PPO activity followed by a decrease to the level close to that of the control plant. Whereas PAL activity had only a slight decrease. After inoculation with X. axonopodis ABB - 2, soluble pro- tein content and free phenolic content in the leave of A. andraenum " Alabamb" increased by 21.76% and 34. 66%, respectively, compared to those of non - inoculated control plant.
出处
《华南师范大学学报(自然科学版)》
CAS
北大核心
2013年第1期79-84,共6页
Journal of South China Normal University(Natural Science Edition)
基金
国家自然科学基金项目(31070003)
广东省植物发育生物工程重点实验室开放基金项目(200802)
关键词
红掌
地毯草黄单胞菌ABB-2
防御相关酶
总可溶性蛋白
游离酚
Anthunium andraenum
Xanthomonas axonopodis ABB -2
defense -related enzymes
free phenoliccontent
soluble protein content