地毯草黄单胞菌ABB-2对红掌防御相关生化指标的影响

Defense-Related Biochemical Changes in the Anthurium Andraenum Plant after Inoculation with a Pathogen Xanthomonas Axonopodis ABB-2

研究了地毯草黄单胞菌(Xanthomonas axonopodis)ABB-2对红掌品种"阿拉巴马"的致病性及其防御相关生化影响.结果表明,红掌对该病菌高度敏感,离体叶片接种第4天、盆栽植株接种第8天后,病叶率达到100%.体叶内超氧化物歧化酶(SOD)、过氧化物酶(POD)、多酚氧化酶(PPO)和苯丙氨酸解氨酶(PAL)活性增加,分别在接种后的第4、6、8和12天达到峰值,较非接种分别对照增加了15.37%、35.63%、51.89%和66.43%;总可溶性蛋白、游离酚含量最大值分别为非接种对照的121.76%和134.66%.

The disease development and defense- related biochemical changes in the leaves of Anthurium andrae- hum cultivar ＇ Alabamb＇ were assayed after inoculation with Xanthomonas axonopodis ABB - 2. Pathogenicity assay revealed that＇ Alabamb＇ was highly sensitive to X. axonopodis. On the day 4 in infection experiment of detached leaf and on the day 8 in infection experiment of pot plant with the X. axonopodis, all inoculated leaves developed disease symptoms of bacterial blight, and the percentage of leaves with lesions reached 100%. The activities of four tested defense- related enzymes, SOD, POD, PPO, and PAL in the leaves of Anthurium ＇ Alabamb＇ increased and reached the peaks on days 4, 6, 8 and 12 after inoculation with X. axonopodis ABB - 2, with the increase values of 15.37%, 36.62%, 46. 68%, and 66.43%, respectively, when compared to those of non- inoculated control plant. After the peaks, SOD and POD activities had a continuous decrease until the level of lower than that of the non - inoculated control plant. And PPO activity followed by a decrease to the level close to that of the control plant. Whereas PAL activity had only a slight decrease. After inoculation with X. axonopodis ABB - 2, soluble pro- tein content and free phenolic content in the leave of A. andraenum ＂ Alabamb＂ increased by 21.76% and 34. 66%, respectively, compared to those of non - inoculated control plant.