摘要
采用16S rDNA序列分析技术对降解五氯酚的微氧颗粒污泥形成过程中真细菌和古细菌的种群多样性和动态变化进行了研究.通过对DGGE主要条带进行序列比对,发现颗粒污泥中真细菌和古细菌都与不可培养的微生物相似性高,微氧颗粒污泥中同时存在好氧菌、微氧菌和厌氧菌.通过比较不同菌的相对数量变化发现五氯酚驯化后的颗粒污泥中产生了一系列对五氯酚降解有利的优势细菌和古菌,如Proteobacteria、Sphingomonas、Methanogenic bacteri-um等.
Both Eubacteria and Archaea community diversity and dynamicity were studied during PCP- degrading micro -aerobic granular sludge cultivation using 16S rDNA ribosomal RNA approach. Main bands of DGGE were compared with sequences. The results showed that Eubacteria and Arehaea community were closely related to un- cultured microorganisms. Compared the relative quantity change of different bacteria, there were aerobic, micro - aerobic and anaerobic bactera in micro - aerobic granular sludge simultaneously. After granular sludge was domesti- cated by PCP, the dominant Eubacteria and Archaea for PCP degradation were related to Proteobacteria, Sphin- gomonas, Methanogenic bacterium and so on.
出处
《华南师范大学学报（自然科学版）》
CAS
北大核心
2013年第1期100-104,共5页
Journal of South China Normal University(Natural Science Edition)
基金
国家自然科学基金项目(20977033)
教育部留学回国人员科研启动基金项目
关键词
五氯酚
微好氧颗粒
16S
RDNA序列
微生物菌落
pentachlorophenol （PCP）
micro -aerobic granules
16S rDNA ribosomal RNA
microbial population
