S-腺苷蛋氨酸对大鼠肝缺血再灌注损伤的保护作用

Protective role of S-ademetionine against ischemic-reperfusion injury in rat liver

目的:观察S-腺苷蛋氨酸(思美泰)对SD大鼠肝缺血再灌注损伤的保护作用。方法:将72只SD大鼠随机均分为3组:假手术组、肝缺血再灌注(IR组)、肝缺血再灌注加S-腺苷蛋氨酸干预组(SAM组),每组24只。假手术组暴露肝门并游离十二指肠韧带,20 min后关腹。IR组和SAM组均以肝脏缺血20 min后再灌注的方式造模。SAM组于术后1 h腹腔注射思美泰100 mg.kg-1.d-1,假手术组和IR组予以等体积生理盐水。各组分别于术后第1、4、7、10天各处死6只大鼠,测定其血清ALT、AST、总胆红素(TB)含量,同时取肝左叶部分组织进行肝脏病理组织学观察。结果:SAM组各个时相点血清ALT、AST、TB水平均明显低于IR组,差异有统计学意义(P<0.01),SAM组肝组织病理学损害明显轻于IR组(P<0.01)。结论:S-腺苷蛋氨酸能够明显降低转氨酶、TB,减轻肝病理损伤,保护肝功能。

Objective： To investigate the protective role of S-ademetionine against ischemic- reperfusion injury in rat liver. Methods： Seventy two healthy male Sprague-Dawle rats were equally randomly divided into three group：sham operation group, hepatic ischemic-reperfusion group （IR group）, and hepatic ischemia-reperfusion plus S-ademetionine group（SAM group）. The animal models were induced by 20 min hepatic ischemia and subsequent reperfusion in IR group and SAM group. One hour after surgery, rats of SAM group were given S-ademetionine （100 mg·kg-1·d-1） byintraperitoneal injection, and rats of sham operation group and IR group were given normal saline at the same volume instead. Six rats from each group were sacrificed on the postoperative day （POD） 1, 4, 7 and 10, respectively, and blood samples were collected and analyzed for the levels of serum AST, ALT, total bilirubin and the histological changes in hepatic tissue were observed. Results： On the POD 1, 4, 7 and 10, the level of ALT, AST, TB were significantly lower in SAM group than those in IR group（P〈 0.01 ） and the pathological changes of liver were significantly slighter in SAM group than in IR group（P〈 0.01 ）. Conclusion： S-ademethionine can significantly reduce aminotrans- ferase and bilirubin, relieve the pathological damage of liver to protect liver function.