脓毒症大鼠体内内源性二氧化硫的分布

Distribution of Endogenous Sulfur Dioxide in Septic Rats

目的探讨内源性二氧化硫(sulfur dioxide,SO2)在脓毒症大鼠肝脏、肾脏、心脏、脑及血清中的分布与水平变化。方法雄性SD大鼠40只,随机分为假手术组(n=8)和脓毒症模型组(n=32);脓毒症模型组大鼠以盲肠结扎穿刺术(cecal ligation and puncture,CLP)制作脓毒症模型。造模后3、6、12及24h处死大鼠,取肝脏、肾脏、心脏、脑组织,并留取血清标本。SO2测定采用酶联免疫吸附试验(ELISA)。结果假手术组大鼠肝脏、肾脏、心脏、脑及血清中均含有一定量SO2,且以心脏组织中的含量最高。与假手术组比较,CLP造模后3h后大鼠肝脏、肾脏、心脏、脑及血清中SO2水平开始升高,12h有统计学意义,24h达到高水平。结论 CLP大鼠体内SO2水平高于正常,可能与脓毒症有一定的相关性。

Objective To investigate the distribution and content of endogenous sulfur dioxide （SOs） in septic rats. Methods Forty SPF male SD rats were randomly divided into control group （ n = 8） with sham operation and experimental group （ n = 32） with sep- sis rat model by cecal ligation and puncture （CLP）. The model group rats were sacrificed after 3h, 6h, 12h and 24h of surgery. Liver, kidney, heart, brain and serum specimens were separated quickly. The concentration of SO2 was detected by enzyme -linked immunosor- bent assay （ELISA）. Results SO2was endogenously generated in rat tissues including liver, kidney, heart, brain and serum. The SO2 content was higher in heart than in other tissues. Compared with the control group, the S02 levels increased at 3h after CLP, then showed gradual increasing tendency to 24h. Conclusion SOs content in heart is higher than that in other organs. The increased production of SOs in sepsis rats suggests that SO2 might be, in our present opinion, involved in the pathogenesis of septic rats.