百会穴局部腺苷A1受体对电针诱导脑缺血耐受的作用

Local adenosine A1 receptors of baihui acupoint mediate cerebral ischemia tolerance induced by Electroacupuncture

目的观察百会穴局部腺苷A1受体（A1R）是否介导电针诱导的脑缺血耐受。方法将60只sD大鼠按随机区组的方法分成5组：假手术组（S组）；模型组（M组）；电针组（E组）；穴位注射A1R激动剂组（CCPA组）；穴位注射二甲基亚砜组（DMSO组），每组12只。采用大脑中动脉阻塞（MCAO）法建立大鼠局灶性脑缺血再灌注损伤模型。E组在造模前2h电针预处理大鼠百会穴；CCPA组和DMSO组分别在造模前2h百会穴注射CCPA（0．1mmol／L）、DMSO各20山。再灌注24h时观察大鼠的神经行为学后立刻处死大鼠，各组6只大鼠行Trc染色评估脑梗死体积，另外各组剩余6只大鼠行免疫印迹（Western blotting）法检测脑组织中Bc1-2蛋白表达量。结果E组与M组相比较神经行为学表现有改善；脑梗死体积从61．72％±7．12％（M组）减小为22．37％±4．42％（E组）；Bcl-2蛋白表达量从0．52％±0．19％（M组）增加为1．07％±0．41％（E组），差异都具有统计学意义（P〈0．05）。CCPA组与M组和DMSO组相比较行为学有改善；脑梗死体积分别为61．72％±7．12％（M组），56．90％±4．7％（DMSO组）减小为25．12％±4．17％（CCPA组）；Bc1-2蛋白表达量分别为0．52％±0．19％（M组），0．56％±0．17％（DMSO组）增加至0．92％±O．37％（CCPA组），差异均有统计学意义（均P〈0．05）。E组和CCPA组行为学表现、脑梗死体积分别为22．37％±4．42％（E组）和25．12％±4．17％（CCPA组），和Bcl-2蛋白表达量分别为1．07％±0．41％（E组）和0．92％±0．37％（CCPA组），差异均无统计学意义。结论电针可以诱导MCAO大鼠的脑缺血耐受；百会穴局部A1R可能介导了电针预处理MCAO大鼠的脑缺血耐受作用。

ischemia tolerance Objective To investigate whether induced by el local A1R of Baihui acupoint mediate cerebral （EA）. Methods Sixty SD rats were randomly divided into five groups, i.e., the sham-operation （S） group, the model group （M）, the electroacupuncture （E） group, the CCPA group and the DMSO group. The focal cerebral ischemia/ reperfusion model was established by middle cerebral artery occlusion （MCAO） in rats. Rats in the E group were received EA pretreatment baihui acupoint at 2 h before established MCAO. The rats in DMSO group and the CCPA group were injected with DMSO （20 td） and CCPA （0. 1 mmol/L） 20into Baihui, respectively,at 2 h before established MCAO. After 24 h reperfusion, the rats＇ behavior, cerebral infarct volume, the cerebral Bcl-2 protein expression were assessed. Results Compared with M group, the rats＇ behavior was improved, the cerebral infarct volume was decreased and the Bcl-2 protein expression was up- regulated （P 〈0. 05） in the E group. Compared with M and DMSO group, the rats＇ behavior was improved, the cerebral infarct volume was decreased and the Bcl-2 protein expression was up-regulated （ P 〈 0. 05 ） in the CCPA group. There were no statistical differences between CCPA and E group. Conclusions EAinduced cerebral ischemia tolerance. Local AIR of Baihui acupoint possible mediate cerebral ischemia tolerance induced by Electroacupuncture.