人类乳头瘤病毒16型早期基因178位(T→G)突变分析

Analysis of the point mutation of high risk HPV16 early gene nt178(T→G)

目的检测宫颈癌患者高危型人类乳头瘤病毒(human papillomavirus,HPV)16型阳性标本中早期基因(E6基因)178位(T→G)点突变情况。方法应用模板指导的染料终止子掺入-荧光偏振检测技术(TDI-FP)方法检测宫颈癌患者高危HPV16型E6基因178位点突变情况,以进一步明确此基因位点突变与宫颈癌变的相关性。结果应用TDI-FP法检测宫颈癌组织中高危HPV16型E6基因178位突变率为32.5%,与癌前病变及慢性宫颈炎患者相比,差异具有统计学意义(χ2=20.623,P<0.01)。结论宫颈癌患者HPV16型E6基因178位(T→G)突变率较高,可能是HPV致癌的一个高危因素。

Objective To detect the point mutation of high risk HPV16 early gene（E6） nt178（T→G） in cervical cancerous tissues.Methods We detected the point mutation of HPV16 E6 nt178 in the detected human papillomavirus（HPV） 16 positive cervical cancerous tissues by TDI-FP method so as to explain the correlation between the point mutation and cervical cancer.Results The HPV16 E6 nt178（T→G） point mutation rate was 32.5% in the present study.The point mutation rate of HPV16 E6 nt178 was significantly different from that of the control group（χ2=20.623,P0.01）.Conclusion The point mutation rate of HPV16 E6 nt178 was higher in our study,which indicates that the point mutation of HPV16 E6 nt178 may promote the malignant process in cervical cancer patients and may be one of the cancer risk factors of HPV.