化学去细胞同种异体神经复合神经生长因子移植后运动功能神经生理学观察

Motor Electrophysiological Experiments on Rats after a Sciatic Nerve Gap Repaired by a Chemically Extracte Acellular Nerve Allograft with Nerve Growth Factor

目的:观察化学去细胞同种异体神经复合神经生长因子(NGF)修复大鼠坐骨神经缺损的神经生理恢复情况。方法:采用药物微球技术制备的NGF微球,与生物纤维蛋白胶混合后,形成NGF复合缓释制剂,作为补充外源性NGF载体。选取30只Wister大鼠制备坐骨神经10 mrn缺损进行神经修复,随机分为A、B、C3组,A组予自体神经反转吻合,B组予化学去细胞异体神经桥接,C组在化学去细胞神经移植段周围注射1 mL的NGF复合缓释制剂。术后16周观察大鼠在运动功能神经生理学恢复的情况。结果:方波刺激移植段近侧神经,均在小腿三头肌上记录到运动诱发电位曲线。A、B、C组的神经移植段运动传导速度分别为(52.6±3.9)、(35.4±3.2)、(47.2±3.8)m/s,A组与C组比较无统计学差异,B组与C组比较差异有统计学意义(P<0.05)。结论:化学去细胞神经同种异体复合NGF缓释制剂移植修复大鼠坐骨神经长段缺损,术后4个月运动传导功能恢复与自体神经移植相似。

Objective： To observe the nerve electrophysiological restoration of a gap made in the sciatic nerve in rat which was repaired by chemically extracted acellular nerve graft with nerve growth factor （NGF）. Methods： The microspheres of NGF were prepared with drug microsphere technology and fixed with the fibrin gels to make the complicated controlled release. The 30 Wister rats were randomly divided into three groups： autografling group （group A）, acellular nerve allografting （group B） and acellular nerve allograft with the double controlled release NGF （group C）. The nerve functional restoration was observed by electrophysiologieal experiments on all the rats at weeksl6 after the operation, including motor evoked potential of the muscular triceps surae and motor conduction velocity of the sciatic grafting nerve segment. Results： Stimulation of the experimented sciatic nerves proximal to the implanted segment resulted in motor evoked potentials recorded from the muscular triceps surae in all rats.The motor conducting velocities of the grafting segment were 52.6 m/s in group A, 35.4m/s in group B, and 47.2m/s in group C. Conclusion： The complicated controlled release drug of NGF can supply the exogenous NGF to improve motor conducting functional restoration in rats.