摘要
目的:探讨急性脑梗死患者血浆总同型半胱氨酸(Hcy)、总半胱氨酸(Cys)、总半胱氨酰甘氨酸(CysGly)、总谷胱甘肽(GSH)、叶酸(FA)和维生素B12(VitB12)水平的变化及其相关性。方法:采用高效液相色谱法检测80例急性脑梗死患者(脑梗死组)和60例健康成人(正常对照组)的血浆Hcy、Cys、CysGly、GSH浓度,同时用放射免疫法测定FA及VitB12水平。脑梗死组血浆Hcy与其它相关硫醇物、FA和VitB12相关性采用Pearson相关系数分析。结果:与正常对照组比较,脑梗死组血浆Hcy、Cys、CysGly水平显著增高,血浆GSH、FA、VitB12水平显著降低(P<0.01)血浆Hcy与Cys浓度呈明显正相关(r=0.6663,P<0.0001),与GSH、FA、VitB12呈明显负相关(r=-0.6099;r=-0.6032;r=-0.5826,P<0.05),与CysGly无明显相关(r=-0.0829,P=0.6870)结论:急性脑梗死患者血浆Hcy以及相关硫醇物发生代谢紊乱,高水平的Hcy、Cys和低水平的GSH、FA、VitB 1 2可能共同参与脑梗死发病机制。
Objective： To explore the association of acute cerebral infarction with plasma levels of homocysteine （Hcy）, cysteine （Cys）, Cysteinylglycine （CysGly）, total glutathione （GSH）, folic acid （FA）, and vitamin B12 （B12）. Methods： Blood samples were collected from 80 cases of acute cerebral infarction patients and 60 health individuals. Plasma levels of Hcy, Cys, CysGly, and GSH were measured by high-performance liquid chromatography, and plasma levels of FA and VitBl2 were measured by radioimmunoassay. The relationships among the plasma levels in the patients were analyzed by Pearson correlation analysis. Results： Plasma levels of Hcy, Cys, and CysGly were significantly increased, but the plasma levels of GSH, FA and VitB 12 were significantly decreased （P 〈 0. 01） in the patients relative to the controls. There was a positive correlation between the plasma levels of Hcy and Cys （r=0.6663,P〈0.0001）. Whereas, negative correlations among the plasma levels Hcy, GSH, FA, and VitB12 were found in the patients （r=-0.6099; r=-0.6032; r=-0.5826, P 〈 0.05） Conclusion： alterations of Hcy and thiol in association with disturbance plasma GSH, FA, and VitB 12 may be involved in the pathogenesis of ischemic stroke
出处
《神经损伤与功能重建》
2013年第1期26-29,共4页
Neural Injury and Functional Reconstruction
