栀子苷的定向分离提取方法建立

Directional isolation and extraction of jasminoidin from aqueous extract of Gardenia jasminoides Ellis

目的:建立栀子苷的定向分离模式,提取栀子药材中的栀子苷。方法:依托以Lipid A为靶点的抗LPS药物筛选平台,应用大孔吸附树脂、膜分离和HPLC等技术对栀子水提液进行针对Lipid A的逐级分离纯化。结果:从栀子水提液中分离出活性化合物栀子苷,其与Lipid A的特异性结合值为112 arc seconds。结论:本研究所建立的栀子苷的定向分离模式,目的性强、效率高,可用于天然药物中抗LPS活性物质的富集与分离纯化。

Objective： To establish the method of directional isolation and extraction of jasminoidin from aqueous extract of Gardenia jasminoides Ellis （GjE）. Methods ： Based on the biosensor technology platform for screening anti - LPS compound which targeted as Lipid A, the aqueous extract of GjE was isolated and purified gradually by using macroporous adsorptive resins, membrane separation and HPLC technology in turn. Results：The jasminoidin was attained form aqueous extract of GjE, the value of specific binding to Lipid A was 112 arc/s. Conclusion： The established directional isolation mode of jasminoidin is more effective than that of traditional method, which is applicable for enriching and purifying anti -LPS active compound from natural medicine.