紫杉醇脂质体对乳腺癌MCF-7细胞生长抑制作用的机制

Mechanism of Paclitaxel Liposome Inhibitory Action on Breast Cancer Cell

目的分析紫杉醇脂质体对乳腺癌MCF-7细胞的生长抑制作用,观察药物作用的时间浓度关系并探究其作用机制。方法应用不同剂量紫杉醇脂质体对MCF-7细胞进行处理,通过MTT法检测细胞存活率,倒置显微镜观察细胞形态,流式细胞分析技术检测细胞凋亡情况及其对细胞周期的影响,Western blot法检测bcl-2蛋白表达情况。结果紫杉醇脂质体对MCF-7细胞有明显的剂量-时间依赖效应。紫杉醇与紫杉醇脂质体均可将MCF-7细胞阻滞在G2/M期,且随药物浓度增加,凋亡细胞所占比例逐渐增加。通过Western blot法检测发现,紫杉醇脂质体作用后MCF-7细胞bcl-2蛋白表达明显下降,bax蛋白表达明显上调,bax/bcl-2比例明显上调。结论与紫杉醇相比,紫杉醇以脂质体对人乳腺癌MCF-7细胞具有同样的抑制作用,其促凋亡机制之一为调控bcl-2及bax蛋白表达。

Objective To study the inhibitory action of paclitaxel liposome to breast cancer cell（MCF-7） and analyze the mechanism through detecting the time-concentration effect of paclitaxel liposome.Methods MCF-7 cells were cultured and treated with different concentration of paclitaxel liposome.The cell viability was analyzed by MTT.Cell morphology was observed inverted microscope.Cell apoptosis and cell cycle arrest status were detected by flow cytometry.The expressions of bcl-2 and bax proteins were detected by Western blot Results The paclitaxel liposome could inhibit MCF-7 cells by time and concentration-dependent effect.The paclitaxel and paclitaxel liposome could block MCF-7 cells at G2/M phase,and cell apoptosis was more obvious along with drug concentration increasing.The Western blots results demonstrated that after using paclitaxel liposome,the expression of bcl-2 protein was obviously decreased,while the expression of bax protein increased.The value of bax/bcl-2 was up-regulated.Conclusion Compared with paclitaxel,paclitaxel liposome can similarly inhibit MCF-7 cells.One of the pro-apoptotic mechanisms is regulating the expression of bcl-2 and bax.