SATB1基因在结直肠癌中的表达及其临床意义

Expression and Clinical Significance of Special AT-rich Sequence-binding Protein in Colorectal Carcinoma

目的检测结直肠癌组织中特异AT序列结合蛋白1(special AT-rich sequence binding protein,SATB1)的表达情况与结直肠癌生物学行为的相关性。方法采用反转录多聚酶链式反应(RT-PCR)法检测39例结直肠癌及相应癌旁对照组织中SATB1mRNA的表达,采用免疫组织化学方法检测39例结直肠癌及相应癌旁对照组织中SATB1的表达,分析SATB1基因表达与患者临床病理因素的相关性。结果癌组织中SATB1蛋白表达阳性率为69.23%(27/39),SATB1 mRNA表达量为(1.178±0.644),均高于癌旁组织,差异有统计学意义(P<0.05)。而SATB1蛋白在结直肠癌组织中的阳性表达率与有无淋巴结转移显著相关(P<0.01),有淋巴结转移其SATB1蛋白阳性表达率为100%,与肿瘤浸润深度和临床TNM分期密切相关(P<0.05)。SATB1mRNA的表达与肿瘤浸润深度、是否淋巴结转移显著相关(P<0.01),与肿瘤临床分期密切相关(P<0.05)。结论 SATB1基因在癌组织中的表达较癌旁组织显著增高表明其可能与结直肠癌的发生有关,而SATB1高表达预示结直肠癌恶性程度高临床分期较晚,可能存在淋巴结转移,预后不良,因此检测SATB1表达可作为临床上筛选结直肠癌高危转移患者、制定治疗方案和判断预后的一个新指标,并有望成为新的治疗靶点。

Objective To detect the expression of special AT-rich sequence-binding protein 1（SATB1） and its correlation to the biological behavior in human colorectal carcinoma.Methods SATB1mRNA and protein were detected in 39 cases of colorectal carcinoma and adjacent non-tumorous tissues by reverse transcriptase polymerase chain reaction（RT-PCR）and Immunohistochemistry respectively.The correlations between SATB1 and clinicopathological parameters were analyzed.Results The expression of SATB1 in colorectal carcinoma tissues was significantly higher than that in adjacent non-tumorous tissues（P0.05）.The positive expression rate of SATB1 was 69.23%（27/39）,and positively correlated to tumorous invasion and clinical stages and metastasis.Over expression of SATB1mRNA was more predominant in patients with deeper invasive colorectal carcinoma（P〈0.05）,and the expression of SATB1mRNA was correlated to the clinical stage and metastasis of the tumor.Conclusion SATB1 expression was associated with the development and metastasis of human colorectal carcinoma,and might serve as an important parameter for determining the clinical diagnosis,treatment and prognosis.