PEBP4基因在食管癌中的表达及其临床意义

Expression and Significance of PEBP4 Protein in Esophageal Cancer

目的探讨磷脂酰乙醇胺结合蛋白4(phosphatidylethanolamine-binding protein 4,PEBP4)基因在食管癌组织中的表达及其与食管癌临床病理的相关性,并探讨其在食管癌侵袭转移过程中的作用。方法采用RT-PCR、免疫组织化学和Western blot方法检测56例食管癌组织中PEBP4mRNA和蛋白的表达情况,采用RNA干扰技术,下调Eca-109人食管癌细胞中PEBP4表达后,观察Eca-109细胞侵袭能力的变化。结果全组56例食管癌患者,PEBP4mRNA和蛋白在食管癌组织中的表达显著高于癌旁相对正常黏膜组织(P<0.05),在淋巴结转移及远处转移阳性患者癌组织中的表达显著高于在淋巴结转移和远处转移阴性患者癌组织中的表达(P<0.05);但PEBP4的表达与食管癌患者的性别、年龄及肿瘤大小及分化程度无关(P>0.05)。下调PEBP4蛋白表达可以显著降低Eca-109细胞的侵袭转移能力(P<0.01)。结论 PEBP4蛋白的过表达可能与食管癌的发生及侵袭转移有关。

Objective To study the expression of PEBP4 in esophageal cancer,and relationship between PEBP4 and the clinical pathologic features of esophageal cancer,and the function of PEBP4 in invasion and metastasis.Method The expressions of PEBP4 mRNA and protein were detected using RT-PCR,immunohistochemistry and Western blot in esophageal cancer tissues and peri-cancer tissues.The change in the invasive ability of human esophageal cancer cell line Eca-109 was detected after knocking down PEBP4 expression using RNA interference.Result The positive mRNA expression rate of PEBP4 in esophageal cancer tissues was significantly higher than that in peri-cancer tissues（P〈0.05）.The positive mRNA expression rate in the carcinoma tissues from patients with positive lymph nodes or distant metastasis was significantly higher than that from patients without lymph nodes or distant metastasis（P〈0.05）.However,expression of PEBP4 was independent of gender,age,tumor size,or tumor differentiation level（P〈0.05）.The results of PEBP4 protein were similar to the PEBP4 mRNA.Invasion and metastasis of Eca-109 cell might significantly be inhibited through down-regulation of PEBP4 protein（P〈0.01）.Conclusion The over-expression of PEBP4 might be related to the carcinogenesis and development as well as metastasis and invasion of esophageal cancer.