Notch2在喉鳞状细胞癌中的表达及意义

Expressions and Significance of Notch2 Protein in Laryngeal Squamous Cell Carcinoma

目的探讨Notch2在喉癌组织及体外培养的喉鳞癌细胞Hep-2中的表达及与喉癌患者临床病理资料之间的关系。方法应用量子点超敏免疫荧光染色法检测95例喉癌组织标本中Notch2蛋白的表达,同时选取31例声带息肉标本作为对照组,结合患者临床病理资料进行分析;制作细胞爬片并进行量子点(QDs)免疫荧光染色检测Notch2蛋白在喉鳞癌细胞Hep-2中的表达。结果 95例喉癌组织中Notch2阳性表达率为92.6%(88/95),其阳性率与声带息肉中的阳性率(32.3%)相比差异均有统计学意义(P<0.01),其表达主要定位于细胞核。Notch2在喉癌组织中的表达与临床分期显著相关(P<0.05):临床分期越高,表达率越高。Notch2在喉癌组织细胞核中表达阳性与T分级、临床分期、病理分级和淋巴结转移显著相关(P<0.05)。喉癌细胞Hep-2中Notch2表达阳性,其表达主要定位于细胞质和胞膜中。结论 Notch2在喉癌的发生发展中起着重要作用,其可能成为治疗喉癌的分子靶点。

Objective To investigate the expressions of Notch2 protein in laryngeal carcinoma cells（Hep-2） and the relationship between the expression and the clinical and pathological data in laryngeal carcinoma.Methods The expressions of Notch2 in 95 cases of laryngeal carcinoma tissue and 31 cases of vocal cord polyps,as well as laryngeal carcinoma cells（Hep-2）,were detected by QDs-IgG conjugate immunofluorescence staining,and the relationship of the expressions of Notch2 to clinical and pathological features of laryngeal carcinoma was analyzed.Results The expression positive rate of Notch2-positive in laryngeal carcinoma was 92.6%（88/95）,and higher than that in vocal cord polyp（32.3%）（P〈0.01）.Notch2 expression was mainly localized in the nucleus.Notch2 expression in laryngeal carcinoma was significantly associated with clinical stage（P〈0.05）.The higher clinical stage had higher expression rate.Notch2 expression in laryngeal carcinoma was significantly related to T classification,clinical stage,histological grade and lymph node metastasis（P〈0.05）.Laryngeal carcinoma cells Hep-2 had the Notch2 expression and mainly was localized in the cytoplasm and enveloped membrane.Conclusion Notch2 played an important role in development of laryngeal carcinoma,and might be a new molecular target for laryngeal carcinoma.