摘要
目的:克隆布鲁氏菌外膜蛋白bp26基因并构建原核表达系统。方法:用聚合酶链式反应技术扩增得到布鲁氏菌bp26基因片段,与PEASY-E1载体链接,转入BL21(DE3)感受态细胞中,经IPTG诱导后,经SDS-PAGE检测重组蛋白表达,用Western blot鉴定目的蛋白的生物活性。结果:DNA测序结果显示,重组质粒bp26基因的插入位点正确,成功构建了重组质粒PEASY-E1-bp26,经IPTG诱导,表达出大小为27kDa的bp26融合蛋白。结论:获得布鲁氏菌外膜蛋白bp26基因片段,并在大肠杆菌中表达出具有生物活性的bp26融合蛋白。
Objective：To clone and construct the recombinant expression plasmid for bp26 gene of Brucella in E. coll. Methods：The bp26 gene of Brucella was amplified from genomic DNA and cloned into PEASY - E1 vecor. The recombinant plasmid was then transformed into E. coli BL21 （ DE3 ）. The expression of bp26 gene was induced by ITPG and the recombinant protein was identified by SDS -PAGE analysis. Results ：The results of DNAs equencing showed that the sequence and cloning site of the inserted bp26 gene were correct, the recombinant expression plasmid PEASY- E1 -bp26 was 27kDa. Conclusion：The bp26 gene of Brucella are cloned and the recombinant bp26 protein are successfully expressed in E. coll.
出处
《内蒙古农业大学学报（自然科学版）》
CAS
北大核心
2012年第4期118-121,共4页
Journal of Inner Mongolia Agricultural University(Natural Science Edition)
基金
内蒙古自然基金(2010MS1104)
