不同激素配比对辣木组培苗生长的影响

Effects of Different Growth Hormone Ratios on Tissue-cultured Plantlet Growth of Moringa oleifera

以辣木嫩梢茎段为外植体,在25种不同激素配比的培养基上进行培养,探讨不同激素配比对辣木组培苗生长的影响,并在6种不同生根培养基上进行生根试验。研究结果表明:在MS+6-BA0.02～0.5mg.L-1+NAA0.02mg.L-1+蔗糖3%的培养基上辣木组培苗生长良好,增殖倍数达5.6～8.1。在1/2MS+NAA0.2mg.L-1+蔗糖2%的培养基上生根率最高达79%。用形态学上端朝上与朝下两种方式接种到生根培养基上均能在形态学下端生根。

Young stems of Moringa Oleifera were selected as explants, and were cultured on substrate using 25 kinds of ratios of growth hormone to study the effects of the hormone ratios on the tissue-cultured shoots growth of Moringa Oleifera. Besides, rooting tests were done on six kinds of substrates. The results showed that the regenerated plant- lets on the substrate of MS＋6-BA 0.02-0.5 mg L-1 ＋NAA 0, 02 mg L-1 ＋3 % sucrose developed best with 5.6 -8.1 times of proliferation. The rooting rate on the substrate of 1/2 MS＋ NAA 0.2 mg L-1 ＋ 2 % sucrose reached 79%. Both morphological top up and top down seeding ways can lead to morphological rooting at the lower end.