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Myo5a特异性片段原核表达重组质粒的构建

Construction of Prokaryotic Expression Recombinant Plasmid of Specific Fragment Human Myo5a
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摘要 为了构建Myo5a特异性片段的原核表达重组质粒PGEX-4T-3/Myo5a,采用PCR扩增出Myo5a基因特异性片段的蛋白编码序列,经BamH I和Xho I双酶切后,将片段插入原核表达载体pGEX-4T-3,构建重组子PGEX-4T-3/Myo5a。最后重组质粒PGEX-4T-3/Myo5a经鉴定完全正确。 To construct prokaryotic expression recombinant plasmid PGEX-4T-3/Myo5a of Myo5a gene, PCR was used to obtain 131bp coding region of Myo5a. The Myo5a gene was digested by restrictive enzyme Barn HI and Xho I. The gene was inserted into the prokaryotic GST fusion protein expression plasmid pGEX-4T-3, to form PGEX-4T 3/ MyoSa. The recombinant plasmid PGEX-4T-3/Myo5a was identified to be correct.
作者 游荷花 沈敬华
机构地区 山西医科大学汾阳学院 内蒙古医学院
出处 《山西农业大学学报（自然科学版）》 CAS 2012年第5期412-414,共3页 Journal of Shanxi Agricultural University(Natural Science Edition)
关键词 Myo5a 原核表达 重组质粒 Myo5a Prokaryotic expression Recombinant plasmid
分类号 R392.33 [医药卫生—免疫学]
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参考文献10

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二级参考文献11

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山西农业大学学报（自然科学版）
2012年 第5期

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