基于叶绿体基因的香蕉遗传多样性研究

Genetic Diversity of Banana Varieties Based on Genes from Chloroplast

以7种不同来源的香蕉为材料,选择2个叶绿体基因组的非编码区序列(即rpl16内含子序列和psaA-ycf3基因间隔序列),进行PCR扩增、克隆转化及测序,采用邻接法(NJ)构建系统进化树,并测算了相对遗传距离。结果表明,巴西蕉与另外6个抗枯萎病香蕉品种间存在一定的遗传差异,但遗传差异程度不大。

Banana is the most important tropical fruit. In recent years the outbreaks ot banana Fusartum writ have great negative impact on banana production. The study of genetic diversity of banana will provide support to genetic improvement of banana for resistance to Fusarium wilt. Seven banana varieties were used as sequencing materials, and 2 non-coding chloroplast DNA sequences （Intron sequence of rpl16 gene and intergenic sequence of psaA-ycf3 genes） in these varieties were amplified by PCR, cloned by TA, sequenced and analysed by bioinformatics to study the genetic diversity of banana. Neighbor joining method was used to establish phylogenetic tree and relative genetic distance measured. Results showed that Baxi banana had different degrees of genetic diversity as against the other 6 wilt-resistant banana varieties but the difference was not significant.