乙型肝炎患者Pre-S1、Pre-S2抗原与HBV-DNA的相关性分析

Correlation analysis between Pre-S1, Pre-S2 antigen and HBV DNA in patients with HBV infection.

目的探讨乙型肝炎病毒(HBV)Pre-S1、Pre-S2抗原与HBV-DNA的相关性。方法对180例标本采用ELISA方法检测前S1抗原、前S2抗原,采用荧光定量PCR方法检测HBVDNA。结果在150例HBVDNA阳性的乙肝患者中,Pre-S1、Pre-S2的阳性率分别为89.33%和86.00%;30例HBVDNA阴性的乙肝患者中,Pre-S1、Pre-S2的阳性率分别为36.67%和26.67%。HBVDNA阳性和阴性患者的Pre-S1、Pre-S2的阳性率差异有统计学意义(P<0.05)。结论随着HBVDNA载量的增加,HBV乙肝患者的Pre-S1、Pre-S2的阳性率逐渐升高。Pre-S1、Pre-S2抗原与HBVDNA载量具有高度相关性,可作为HBVDNA检测的有效补充。

Objective To explore the relationship between Pre-S1, Pre-S2 and HBV-DNA. Methods Sam-ples of 180 patients infected with HBV were collected and measured for Pre-S1 antigen and Pre-S2 antigen by ELISA. The HBV-DNA was measured by qRT-PCR. Results Among the 150 patients positive for HBV DNA, the positive rates of Pre-Sland Pre-S2 were 89.33% and 86.00%, respectively. Among the 30 patients negative for HBV DNA, the positive rates of Pre-S1 and Pre-S2 were 36.67% and 26.67%, respectively. There was statistically significant dif-ference between the positive rate of Pre-S1 and Pre-S2 antigens in both positive and negative HBV DNA patients （P〈0.05）. Conclusion The positive rate of Pre-S1 and Pre-S2 in HBV patients increases with the increasing of HBV DNA capacity. Pre-S1, Pre-S2 antigens showed significant correlation with HBV DNA capacity, which can be used as complement for HBV DNA detection effectively.